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NME1 (Nucleoside Diphosphate Kinase A, Granzyme A-activated DNase, GAAD, Metastasis Inhibition Factor nm23, NB, NBS, NDPKA, NDPK-A, NDP Kinase A, NM23, nm23-H1, NM23-H1, Tumor Metastatic Process-associated Protein)

Cat no: N7100-61

NME1 (Nucleoside Diphosphate Kinase A, Granzyme A-activated DNase, GAAD, Metastasis Inhibition Factor nm23, NB, NBS, NDPKA, NDPK-A, NDP Kinase A, NM23, nm23-H1, NM23-H1, Tumor Metastatic Process-associated Protein)

NME1 (NM23-H1) is a 19-20kD member of the NDK family of enzymes. NME1 is ubiquitous in expression, and performs multiple functions. It forms disulfide-linked homohexamers, and heterohexamers with NME2, generating a nucleoside diphosphate kinase that catalyzes a phosphoryl transfer from ATP to a nucleoside diphosphate. It also shows His and Ser/Thr protein kinase activity, and forms covalent linkages with molecules diverse as p53 and STRAP. It is found both intracellularly, and in blood at ng/mL concentrations. Human NME1 is 152aa in length, contains one NDP kinase domain (aa5-134) and shows acetylation at Ala2 and Lys56, plus phosphorylation at Tyr52, Thr94, Ser122 and Ser125. Human NME1 shares 89% aa identity with human 17-18kD NME2, and 94% aa identity with mouse NME1. A second H1 isoform named M23-H1B with 25 additional aa at the N-terminus has also been described. \n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.5ug/ml \nOptimal dilutions to be determined by the researcher.\n\nRecommended Secondary Antibodies:\nI1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo\nI1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

N7100-61

Size

100ug

Applications

WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a

Additional Info

Recognizes human NME1 at ~20kD. Does not crossreact with recombinant human NME2.

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