NR1, CT (NMDA (N-methyl-D-aspartate) Receptor subunit NR1)

NR1 along with the NR2 subunit forms a heterodimer that make up the NMDA receptor. The NMDA receptor is characterized as a ionotropic receptor for glutamate. As the name suggests, (NMDA (N-methyl D-aspartate) is an agonist for the receptor. A distinct characteristic of the NMDA receptor is that it is both ligand-gated and voltage-dependent based on its sensitivity to magnesium. Activated NMDA receptors result in the opening of an ion channel that is nonselective to cations. The result is the flow of Na+ and small amounts of Ca2+ ions into the cell and K+ out of the cell. Calcium flux by the NMDARs has been implicated in synaptic plasticity, a mechanism in the cell for learning and memory.\n\nApplications: \nSuitable for use in ELISA, Immunocytochemistry, Immunoprecipitation and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nImmunocytochemistry: Shows positive staining in QT-6 cells, transfected to express NR1, which were fixed with 4% paraformaldehyde/4% sucrose in PBS and in nontransfected cultured rat neurons.\nImmunoprecipitation: 4ug immunoprecipitated NR1 from 500ug of rat brain microsomal protein preparation. \nWestern Blot: 0.5-2ug/ml. Detects NR1 from 20ug of rat brain microsomal protein preparation. Rat brain microsomal preparation was resolved by electrophoresis, transferred to nitrocellulose and probed with N5380-05 (0.5ug/ml). Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system. Note: Do not boil the microsomal preparation. Incubate at RT for 30-45 minutes.\nOptimal dilutions to be determined by the researcher.\n\nRecommended secondary antibodies:\nI1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo\nI1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

N5380-05

Size

200ug

Applications

ELISA, ICC, IP, WB

Hosts

Mouse

Reactivities

Rat

Form

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG

References

1. Hisatsune, C., et al., J. Biol. Chem. 272: 20805-20810, 1997. 2. Leonard, A.S. and J.W. Hell, J. Biol. Chem. 272: 12107-12115, 1997. 3. Raymond, L.A., et al., J. Physiol. Paris. 88: 181-192, 1994. 4. Tingley, W.G., et al., Nature 364: 70-73, 1993.5. Tingley, W.G., et al., J. Biol. Chem. 272: 5157-5166, 1997.

Additional Info

Recognizes rat NR1 (Mr 130kD). An additional band (Mr 200kD) may be detected at higher concentrations. The epitope maps to a region (834-864) common to all NR1 isoforms and so should crossreact with all NR1 isoforms. Species sequence homology: human and mouse.