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NR1, phosphorylated (Ser896) (NMDA Receptor, N-methyl-D-aspartate Receptor)

Cat no: N5380-08

NR1, phosphorylated (Ser896) (NMDA Receptor, N-methyl-D-aspartate Receptor)

NR1 (NMDA1or NMDAR1, also known as GRIN1) along with the NR2 subunit forms a heterodimer that make up the NMDA receptor. The NMDA receptor (NMDAR) is characterized as a ionotropic receptor for glutamate. As the name suggests, (NMDA (N-methyl D-aspartate) is an agonist for the receptor. A distinct characteristic of the NMDA receptor is that it is both ligand-gated and voltage-dependent based on its sensitivity to magnesium. Activated NMDA receptors result in the opening of an ion channel that is nonselective to cations. The result is the flow of Na+ and small amounts of Ca2+ ions into the cell and K+ out of the cell. Calcium flux by the NMDARs has been implicated in synaptic plasticity, a mechanism in the cell for learning and memory.\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested. \n\nRecommended Dilutions: \nWestern Blot: 1ug/ml detects NR1 on 10ug of lambda phosphatase untreated and treated rat microsomal brain tissue lysates.\nImmunohistochemistry: 1:25 detects NR1 in normal rat hippocampus tissue.\nOptimal dilutions to be determined by the researcher. \n\nPositive Control: \nLambda phosphatase untreated and treated rat microsomal brain tissue lysates\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

N5380-08

Size

100ug

Applications

FC, IHC, WB

Hosts

Rabbit

Reactivities

Rat

Form

Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150 mM sodium chloride, 0.05% sodium azide.

P Type

Pab

Purity

Purified by affinity chromatography.

Isotype

IgG

Additional Info

Recognizes rat NR1 when phosphorylated at Ser896. Species sequence homology: human, mouse 100%.

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