PACAP (Pituitary Adenylate Cyclase Activating Polypeptide)

Vasoactive intestinal peptide (VIP) is a 28 amino acid peptide (human, chr 6q26-q27). Expressed and secreted by neurons innervating primary and secondary immune organs such as lymph nodes with a Mol.wt of 20kD. VIP is a potent neurotrophic factor causes vasodilation, lowers arterial blood pressure, and relaxes the smooth muscle of trachea, stomach and gall bladder. VIP also modulates several T-lymphocyte activities including motility, cytokine production, proliferation and apoptosis, VIP exerts its biological activity by binding to two closely related class II G-protein-coupled receptors VPAC-1 and VPAC-2 beside this VIP has its own receptors VIPR1&2 (Vasoactive Intestinal Polypeptide Receptor 1&2) and VIPRRP (VIP receptor-gene repressor protein). VIP shows similarities to glucagon, secretin and gastric inhibitory peptide (GIP) as such it has been considered a member of the glucagon-secretin family.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 0.5-1ug/ml; Control peptide can be used to coat ELISA plates at 1ug/ml. See P1774-95A.\nWestern Blot: 1-10ug/ml using ECL technique.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

P1774-95

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% BSA, before the addition of glycerol to 40%.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Bodner,M (1985) PNA,82, 3548-3551 2. Delgado, M (2001) Nature Med, 7, 563-568 3. Hamelink, C et al(2002) PNA Sci,99, 461-466 4. Hashimoto, H. et al(2001) PNA sci 98,13355-60; 5. Gotoh, E.(1998) Biochem. Int, 17, 555-562

Additional Info

Recognizes human PACAP. Species Sequence Homology: mouse - 83%; rat - 81%.