PAM (Peptidyl-Gly alpha-Amidating Monooxygenase)

PAM (Peptidyl-Gly alpha-amidating monooxygenase) is a dual-function, 95-110kD protein that belongs to both the lyase and monooxygenase enzyme families. It is expressed in a variety of endocrine and exocrine glands and catalyzes the conversion of C-terminal Gly into amidated residues. Human ProPAM is a 953aa type I transmembrane glycoprotein. It contains an 833 aa extracellular domain (ECD) (aa31-863) and an 86 aa cytoplasmic tail. The ECD shows one PHM/monooxygenase region (aa40-347) and a PAL/lyase domain (aa495-817). Both regions are enzymatically active. Alternative splicing may generate a soluble form (aa31-828), and remove aa388-494 that link the two embedded enzyme segments. Over aa 1-817, human PAM is 91% aa identical to mouse PAM.\n\nApplications: \nSuitable for use in Direct ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nDirect ELISA: 0.5-1ug/ml. The detection limit is ~0.2ng/well.\nWestern Blot: 0.1-0.2ug/ml. The detection limit is ~5ng/lane under non-reducing and reducing conditions.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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