PDCD4, phosphorylated (Ser457) (Programmed Cell Death 4, Neoplastic Transformation Inhibitor, H731, MGC33046, MGC33047, Nuclear Antigen H731, OTTHUMP00000020483, Protein 197/15a, RP11 348N5.4)

Programmed cell death 4 (Pdcd4) is a novel tumor suppressor. Pdcd4 directly inhibits the helicase activity of eukaryotic translation initiation factor 4A (eIF4A), a component of the translation initiation complex. Pdcd4 also suppresses the transactivation of activator protein-1 (AP-1)-responsive promoters by c-Jun. Pdcd4 contains two Akt phosphorylation sites, one at Ser67 and the other at Ser457. The phosphorylation of Pdcd4 by Akt causes nuclear translocation of Pdcd4 and a significant decrease in the ability of Pdcd4 to interfere with the transactivation of AP-1 responsive promoters by c-Jun.\n\nApplications: \nSuitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

P3120-68H

Size

100ug

Applications

ELISA, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Xen/Amph

Form

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

1. Jansen AP, Camalier CE, Colburn NH (2005) Epidermal expression of the translation inhibitor programmed cell death 4 suppresses tumorigenesis. Cancer Res. 65(14):6034-6041.\n2. Palamarchuk A, Efanov A, Maximov V, Aqeilan RI, Croce CM, Pekarsky Y (2005) Akt phosphorylates and regulates Pdcd4 tumor suppressor protein. Cancer Res. 65 (24):11282-11286.

Additional Info

This product was affinity purified from monospecific antiserum by immunoaffinity chromatography using phospho-peptide coupled to agarose beads followed by solid phase adsorption against nonphospho-peptide. This antibody is specific for human Pdcd4 protein phosphorylated at Ser457. A BLAST analysis was used to suggest crossreactivity with Pdcd4 from human, mouse, rat and Xenopus based on 100% homology with the immunizing sequence. Cross-reactivity with Pdcd4 from other sources has not been determined. Species Crossreactivity: Reactive with human, mouse, rat and xenopus, based on 100% homology with immunogen.