PDE1B pAb

cAMP-hydrolyzing cyclic nucleotide phosphodiesterase (PDE) catalyzes hydrolysis of the cyclic nucleotides cAMP and cGMP to the corresponding nucleoside 5-prime-monophosphates. PDEs are key enzymes in signaling pathways that influence smooth muscle tone regulation. The PDE1 family are calmodulin-dependent (CaM-PDEs) that undergo stimulation through a calcium-calmodulin complex. Human PDE1B (PDE1B1) protein is present in neuronal cells of the cerebellum, hippocampus, and caudate, and lymphoblastoid lines, such as RPMI-8392 cells. PDE1B may participate in learning, memory, and regulation of phosphorylation of DARPP-32 in dopaminergic neurons. A splice variant known as PDE1B2 encodes a 516-amino acid protein and diverges from PDE1B1 by the replacement of the first 38 residues with an alternative 18 residues. The human PDE1B gene maps to chromosome 12q13, contains 13 exons, and encodes a 536 amino acid protein.

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SPECIFICATIONS

Catalog Number

BS6688

Size

100ug/100ul

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

liquid

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Antigen

Recombinant full length Human PDE1B.

Species

PDE1B pAb detects endogenous levels of PDE1B protein.

Dilutions

WB: 1:500~1:2000\nIHC: 1:50~1:200

Swiss Prot

Q01064

Storage Temp

Store at 4 degrees C short term. Aliquot and store at -20 degrees C long term. Avoid freeze-thaw cycles.

Concentration

1ug/ul

Storage Buffer

1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.

Additional Info

For research use only, not for use in diagnostic procedure.

Molecular Weight

~ 65 kDa

Alternative Names

5''-cyclic nucleotide phosphodiesterase 1B; 63 a Cam PDE; 63 a Cam-PDE; Calcium/calmodulin dependent 3' 5' cyclic nucleotide Phosphodiesterase 1B; Calcium/calmodulin stimulated cyclic nucleotide phosphodiesterase; Calcium/calmodulin-dependent 3''; Calcium/calmodulin-dependent 3'5'-cyclic nucleotide Phosphodiesterase 1B; Calmodulin stimulated phosphodiesterase PDE1B1; Cam PDE 1B; Cam PDE1B; Cam-PDE 1B; PDE 1B; PDE1B; PDE1B; PDE1B1; PDES 1B; PDES1B; Phosphodiesterase 1B; Phosphodiesterase