PECAM-1/CD31 BioAssay(TM) ELISA Kit, Mouse

Sandwich ELISA quantitative detection kit for Mouse PECAM-1/CD31 in cell culture supernates, serum and plasma (heparin, EDTA).\n\nPECAM-1 is an important target GP in DITP.1 Platelet/endothelial cell adhesion molecule-1 (PECAM-1) is a 130kD member of the Ig gene superfamily that is expressed on the surface of circulating platelets, monocytes, neutrophils, and selective T-cell subsets.2 Platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31) is a member of the immunoglobulin (Ig) superfamily that has distinctive features of an immunoreceptor based upon its genomic structure and the presence of intrinsic immunoreceptor tyrosine inhibitory motifs (ITIMs) in its ligand binding polypeptide.3 PECAM-1 has recently been shown to contain functional immunoreceptor tyrosine-based inhibitory motifs (ITIMs) within its cytoplasmic domain, and co-ligation of PECAM-1 with the T-cell antigen receptor (TCR) results in tyrosine phosphorylation of PECAM-1, recruitment of Src homology 2 domain-containing protein tyrosine phosphatase-2 (SHP-2), and attenuation of TCR-mediated cellular signaling.4 The standard used in this kit is the product of gene recombinant expression (extracellular part), consisting of 563 amino acids (E28-K590) with the molecular weight of 65.5kD. After glycosylating, its molecular weight changes to 91-100kD.\n\nAssay Range:\n156pg/ml-10000pg/ml\n\nSensitivity:\n< 10 pg/ml\n\nCrossreactivity:\nThere is no detectable cross-reactivity with other relevant proteins.\n\nUniprot ID:\nQ08481\n\nEntrez Gene ID:\n18613\n\nKit Components:\n96-well plate pre-coated with anti-PECAM-1/CD31, Mouse\nLyophilized recombinant PECAM-1/CD31, Mouse standard, 10ng/tube, 2Ea\nUSB Cat No Kit Component\n143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)\n143697 Sample diluent buffer, 30ml\n143698 Antibody diluent buffer, 12ml\n143699 ABC diluent buffer, 12ml\n143700 TMB color developing agent, 10ml\n143701 TMB stop solution, 10ml\n\nStorage and Handling:\nStore at 4 degrees C for 6 months, at -20 degrees C for 12 months. Avoid multiple freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

144011

Size

96Tests

References

1. Kroll, H., Sun, Q.-H., Santoso, S. Platelet endothelial cell adhesion molecule-1 (PECAM-1) is a target glycoprotein in drug-induced thrombocytopenia. Blood 96: 1409-1414, 2000.\n2. Varon D, Jackson DE, Shenkman B, Dardik R, Tamarin I, Savion N, Newman PJ. Blood. 1998 Jan 15;91(2):500-7.\n3. Jackson DE. FEBS Lett. 2003 Apr 10;540(1-3):7-14. Review.\n4. Newman DK, Hamilton C, Newman PJ. Blood. 2001 Apr 15;97(8):2351-7.\n