Peptone Water is a broth medium used for the detection of indole and carbohydrate fermentation patterns of non-fastidious organisms. Peptone Water is a minimal growth medium which is used for determining carbohydrate fermentation capabilities.
Recommended Dilutions:
Carbohydrate Fermentation Study: 1% final carbohydrate concentration with phenol red (0.018g/L) indicator and Durham tube. After incubation a yellow broth with or without gas bubbles indicates a positive fermentation reaction.
Indole Test: Place an indole test strip 10mm above a 24-48 hr. broth culture. Reincubate for 5-30 minutes. A violet red color on the test strip indicates a positive reaction.
Storage and Stability:
Store unopened medium at 2-30 degrees C. Store opened medium container in a low humidity environment at 2-30 degrees C. Store liquid medium at 4 degrees C. The powder medium should be discarded if it is not free flowing or if the color has changed from the original light beige color.
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