Phosphate Assay Kit, BioAssay(TM)

Description:\nPhosphate (Pi) is one of the most important ion species in nature. Phosphate is present in all biological systems. It is a major constituent in minerals and fertilizers, and is a component of industrial wastewater. Thus accurate determination of phosphate concentration finds numerous applications in pharmacology, biomedical research, clinical chemistry, industrial process monitoring and environmental monitoring.\nSimple, direct and automation-ready procedures for measuring phosphate concentration in biological and environmental samples are becoming popular. Phosphate assay kit is designed to measure phosphate ion directly in samples without any pretreatment. The improved Malachite Green method utilizes the malachite green dye and molybdate, which forms a stable colored complex specifically with inorganic phosphate. The intensity of the color, measured at 620nm, is directly proportional to the phosphate concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw samples.\n\nKey Features:\nSensitive and accurate. Linear detection range 0.30uM (0.0028mg/dl) to 50uM (0.47mg/dl) phosphate in 96-well plate assay.\nSimple and high-throughput. The procedure involves addition of a single working reagent and incubation for 30 min. Can be readily automated as a high-throughput assay for thousands of samples per day.\nImproved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Assays can be executed in cuvet or 96-well plate.\nLow interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples i.e., in the presence of lipid, protein and minerals.\n\nApplications:\nDirect Assays: Pi in serum, urine, saliva, sweat, tissue culture etc.\nDrug Discovery/Pharmacology: effects of drugs on Pi metabolism.\nFood and Beverages: Pi determination.\nEnvironment: Pi determination in water, soil and fertilizer.\n\nKit Contents (500 tests in 96-well plates):\nReagent: 50ml\nPi standard: 14ml 0.28mg/dl (30uM)\nBlank Control: 14ml\nStorage conditions. Store Reagent, standard and blank control at 4 degrees C. Shelf life: 12 months.\nPrecautions: reagents are for research use only. Normal precautions for laboratory reagents should be exercised while using the reagents. Please refer to Material Safety Data Sheet for detailed information.

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SPECIFICATIONS

Catalog Number

P4071-72

Size

500Tests

References

1. Cogan, E.B., et al., Anal Biochem. 271(1): 29-35 (1999). 2. Ekman, P. & Jager, O., Anal. Biochem. 214(1): 138-141 (1993). 3. Fisher, D.K. & Higgins, T.J., Pharm Res. 11: 759-763 (1994).