Pin1, Recombinant, Human (Peptidyl-prolyl cis/trans Isomerase NIMA-interacting 1, PPIase)

Human Pin 1 is a peptidyl-prolyl cis/trans isomerase (PPIase) that interacts with NIMA and essential for cell cycle regulation Pin1 is nuclear PPIase containing a WW protein interaction domain, and is structurally and functionally related to Ess1/Ptf1, an essential protein in budding yeast. PPIase activity is necessary for Ess1/Pin1 function in yeast. Pin1 is thus an essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Substrates of Pin1 include the mitotic regulators (Cdc25 phosphatase and NIMA ,PLK I, Wee, and Myt1 kinases), several transcription factors like beta-Catenin, c-Jun, and the tumor suppressor protein p53 , and some specific proteins like the RNA Pol II, the cytoskeleton protein tau, and the G1/S protein Cyclin D1.\n\nDescription: \nRecombinant Human PPIase produced in E.Coli is a single,non-glycosylated polypeptide chain containing 163 amino acids & having a molecular mass of 18.2kD.\n\nAA Sequence: \nMADEEKLPPG WEKRMSRSSG RVYYFNHITN ASQWERPSGN SSSGGKNGQG EPARVRCSHL LVKHSQSRRP SSWRQEKITR TKEEALELIN GYIQKIKSGE EDFESLASQF SDCSSAKARG DLGAFSRGQM QKPFEDASFA LRTGEMSGPV FTDSGIHIIL RTE\n\nStorage and Stability: \nFor long-term storage, add a carrier protein (0.1% HSA or BSA), aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

P4190-10

Size

5ug

Form

Supplied as a liquid in 20mM Tris-HCl, pH 7.5, 0.1M sodium chloride, 5mM DTT, 20% glycerol.

Purity

(same/more than) 95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.

References

1. Exploring the Molecular Function of PIN1 by Nuclear Magnetic Resonance. Curr Protein Pept Sci 2006 Jun;7(3):179-94 2. Nanomolar inhibitors of the peptidyl prolyl cis/trans isomerase pin1 from combinatorial Peptide libraries. J Med Chem 2006 Jun 1;49(11):3430 3. Expression of Pin1 and Ki67 in cervical cancer and their significance. J Huazhong Univ Sci Technolog Med Sci 2006;26(1):120-2 4. Association study of the PIN1 gene with Alzheimer's disease. Neurosci Lett 2006 Jul 24;402(3):259-61 5. Negative regulation of interferon-regulatory factor 3-dependent innate antiviral response by the prolyl isomerase Pin1. Nat Immunol 2006 Jun;7(6):598-605 6. Comparative analysis of enzyme activities and mRNA levels of peptidyl prolyl cis/trans isomerases in various organs of wild type and Pin1-/- mice. FEBS Lett 2006 May 29;580(13):3237-45