Plastin L (PLSL, L Plastin, L-Plastin, LC64P, Lymphocyte Cytosolic Protein 1, LCP1, LCP-1, Plastin 2, Plastin-2)

Highly conserved and widely expressed plastin proteins comprise a subset of actin-binding proteins that include proteins that promote actin bundling. Three plastins exhibiting differential expression are found in mam-mals and include L-plastin, T-plastin and I-plastin. T-plastin (plastin-3) is found in cells of most solid tissues while I- plastin (plastin-1) is expressed specifically in kidney, colon and small intestine (1-3). L-plastin (plastin-2) or LCP1 \n(lymphocyte cytosolic protein 1) is mainly expressed in hematopoietic cells and non-hematopoietic tumors, including increased expression correlated with metastatic progression \nin colon cancer cell lines (4). Overexpression of LCP1 in premetastatic cancer cell lines induces invasion and loss of E-cadherin expression, which is characteristic of metastatic cancer cell lines (5). LCP1 becomes phosphorylated at Ser5 upon stimulation through the T-cell receptor/co-receptor complex (TCR/CD3) in association with the CD2 cell adhe-sion molecule or the CD28 receptor (6). Phosphorylation at Ser5 increases LCP1 ability to bind F-actin and increase cell motility (7,8). \n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000 Incubate membrane with diluted antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight. \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

P4268-01B

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in 10mM sodium HEPES pH 7.5, 0.1%, 150mM NaCl, 100ug/ml BSA and 50% glycerol.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

References

1. Lin, C.S. et al. (1993) J Biol Chem 268, 2781-92, 2. Lin, C.S. et al. (1994) Mol Cell Biol 14, 2457-67, 3. Delanote, V. et al. (2005) Acta Pharmacol Sin 26, 769-79, 4. Otsuka, M. et al. (2001) Biochem Biophys Res Commun 289, 876-81, 5. Foran, E. et al. (2006) Int J Cancer 118, 2098-104, 6. Wabnitz, G.H. et al. (2007) Eur J Immunol 37, 649-62, 7. Janji, B. et al. (2006) J Cell Sci 119, 1947-60, 8. Klemke, M. et al. (2007) Int J Cancer 120, 2590-9.

Additional Info

Recongnizes endogenous levels of human total LCP1 protein. Species Crossreactivity: Mouse. Species sequence homology: rat.