POLQ (DNA Polymerase theta, DNA Polymerase eta, POLH)

Cat no: P4448-45

Supplier: United States Biological

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Has a DNA polymerase activity on nicked double-stranded DNA and on a singly primed DNA template. The enzyme activity is resistant to aphidicolin, and inhibited by dideoxynucleotides. Exhibites a single-stranded DNA-dependent ATPase activity. Could be involved in the repair of interstrand cross-links. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: ELISA: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Catalogue number:	P4448-45
Reactivities:	Human
Hosts:	Goat
Applications:	ELISA
Size:	100ug
Form:	Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
P type:	Pab
Purity:	Purified by immunoaffinity chromatography.
References:	1. Kawamura K, Bahar R, Seimiya M, Chiyo M, Wada A, Okada S, Hatano M, Tokuhisa T, Kimura H, Watanabe S, Honda I, Sakiyama S, Tagawa M, O-Wang J. DNA polymerase theta is preferentially expressed in lymphoid tissues and upregulated in human cancers. Int J Cancer. 2004 Mar;109(1):9-16.
Additional info:	Recognizes DNA polymerase theta. Species sequence homology: Human.

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POLQ (DNA Polymerase theta, DNA Polymerase eta, POLH)

Cat no: P4448-45

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