PPARg, Recombinant, Human (Peroxisome Proliferator Activated Receptor, gamma, HUMPPARG, NR1C3, PPARG1, PPARG2)

The peroxisome proliferator activated receptors (PPARs) are ligand-activated transcription factors within the nuclear receptor superfamily which play important roles in adipogenesis, glucose homeostasis and inflammation. Three different isotypes can be distinguished: alpha, beta and gamma. PPAR-g is mainly expressed in adipose tissue and to lesser extent in colon, the immune system and the retina. Whereas PPARa operates in the catabolism of fatty acids in the liver, PPARg influences the storage of fatty acids in the adipose tissue and plays a role in adipocyte differentiation. PPARg binds to DNA as a heterodimer with retinoid X receptor (RXR), activating expression of target genes after binding ligand. \n\nRecombinant Human Peroxisome Proliferator Activated Receptor Gamma expressed in E. coli is fused to an amino terminal hexahistidine tag and has a molecular weight of 59.2kD.\n\nBiological Activity:\nSpecific activity is 3-4 units/ml obtained by measuring the increase of NADPH in absorbance at 340nm resulting from the reduction of NADP. In the coupled mode, one unit will produce 1.0 umole of NADPH per minute as glucose is phosphorylated by ATP at pH 7.4 at 30C\n\nStorage and Stability: \nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

P5505-01D

Size

2ug

Form

Supplied as a liquid in PBS, 50% glycerol, 0.01% sodium azide.

Purity

(same/more than)90% (SDS-PAGE and RP-HPLC)

References

1. Peroxisome proliferator-activated receptor gamma agonists in renal disease. Eur J Endocrinol 2006 May;154(5):613-21 2. Bioassay for the identification of natural product-based activators of peroxisome proliferator-activated receptor-gamma (PPARgamma): the marine sponge metabolite psammaplin A activates PPARgamma and induces apoptosis in human breast tumor cells. J Nat Prod 2006 Apr;69(4):547-52 3. Polymorphisms of the peroxisome proliferator-activated receptor-gamma and its coactivator-1alpha genes in Chinese women with polycystic ovary syndrome. Fertil Steril 2006 May;85(5):1536-40 4. Adipocyte-type fatty acid-binding protein as inter-compartmental shuttle for peroxisome proliferator activated receptor gamma agonists in cultured cell. Biochim Biophys Acta 2006 Feb;1761(2):172-81 5. Peroxisome proliferator-activated receptor gamma ligands stimulate myeloid differentiation and lipogenensis in human leukemia NB4 cells. Dev Growth Differ 2006 Apr;48(3):177-88 6. Fish oil regulates adiponectin secretion by a peroxisome proliferator-activated receptor-gamma-dependent mechanism in mice. Diabetes 2006 Apr;55(4):924-8