PrCP, Active, Recombinant, Human (Lysosomal Pro-X Carboxypeptidase, Angiotensinase C, Lysosomal Carboxypeptidase C, Proline Carboxypeptidase, Prolylcarboxypeptidase, PCP)

PrCP is a lysosomal prolylcarboxypeptidase, which cleaves C-terminal amino acids linked to proline in peptides such as angiotension II, III and des-Arg9-bradykinin. The cleavage occurs at acidic pH, but the enzyme activity is retained with some substrates at neutral pH. This enzyme has been shown to be an activator of the cell matrix-associated prekallikrein. The importance of angiotension II, one of the substrates of this enzyme, in regulating blood pressure and electrolyte balance suggests that this gene may be related to essential hypertension. Alternatively spliced transcript variants encoding distinct isoforms have been observed.\n\nSource:\nRecombinant corresponding to aa31-496 from human PrCP, fused to His-tag at C-terminal, expressed in HEK293 cells.\n\nMolecular Weight:\n~53kD\n\nApplications: \nSuitable for the cleavage of C-terminal amino acids linked to proline in peptides such as angiotension II, III and des-Arg9-bradykinin. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and store at -70 degrees C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

137361

Size

10ug

Form

Supplied as a liquid in 40mM Tris pH 8.0, 110mM sodium chloride, 2.2mM potassium chloride, 20% glycerol, 200mM imidazole.

Purity

Purified (~50%)

References

1. Tan, F., et al. 1993. J Biol Chem 268 (22): 16631-8. 2. Yang, H.Y., et al. 1968. Nature 218 (5148): 1224-6. 3. Wang, L., et al. 2006. Am. J. Obstet. Gynecol. 195 (1): 162-71.