PRDX6, SO3 (1-Cys Peroxiredoxin, 1-Cys PRX, 24kD Protein, Acidic Calcium-independent Phospholipase A2, aiPLA2, Antioxidant Protein 2, Liver 2D Page Spot 40, Non-selenium Glutathione Peroxidase, NSGPx, Red Blood Cells Page Spot 12, AOP2, KIAA0106)

Peroxiredoxin VI (Prx VI, 1-Cys Prx) is a member of Peroxiredoxin Family, an antioxidant enzyme that detoxifies reactive oxygen species and has a cysteine at their active site. Six isoforms (Prx I to VI) of Prx exist in all eukaryotic cells. These isoforms are classified into three subgroups (2-Cys, atypical 2-Cys, and 1-Cys). Prx VI modulates various receptor-signaling pathways and protects cells from cell death induced by oxidative stress. The active site cysteine(Cys47) is oxidized to cysteine sulfenic acid(Cys47-SOH) by H2O2. However, the resulting Cys47-SOH does not form a disulfide bond because of unavailability of another Cys-SH nearby. It can be reduced by nonphysiological thiols such as DDT but is not transformed by Thioredoxin/Thioredoxin Reductase or GSH. Occasionally, the sulfenic intermediate is hyperoxidized to sulfinic or sulfonic acid, resulting in inactivation of peroxidase activity. Involved in redox regulation of the cell. Can reduce H2O2 and short chain organic, fatty acid, and phospholipid hydroperoxides. May play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury.\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1:2000\nImmunohistochemistry (Paraffin): 1:200\nOptimal dilutions to be determined by the researcher.\n\nPositive Controls:\nWestern Blot: HeLa cell treated with H2O2\nImmunohistochemistry: Human pancreas and testis\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

P3352-32N

Size

100ul

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in HEPES, 0.15M sodium chloride, 0.01% BSA, 0.03% sodium azide, 50% glycerol.

P Type

Pab

Purity

Purified

Isotype

IgG

References

1. Chae, H.Z., et al (1994) Proc.Natl. Acad. Sci. USA 91:7017-7021. 2. Choi, H.J. et al (1998) Nat. Struct. Biol 5:400-406. 3. Fisher, A.B., et al (1999) J. Biol. Chem. 274:21326-21334. 4. Kang, S.W. et al (1998) J. Biol. Chem. 273:6303-6311. 5. Seo, M.S., (2000) J.Biol. Chem. 275:20346-20354.

Additional Info

Recognizes Peroxiredoxin VI, SO3. Species Crossreactivity: human