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PRL-1, aa2-173, catalytic domain, Recombinant, Human (Protein tyrosine phosphatase, Phosphatase of regenerating liver-1)

Cat no: P9003-68

PRL-1, aa2-173, catalytic domain, Recombinant, Human (Protein tyrosine phosphatase, Phosphatase of regenerating liver-1)

Catalytic domain, (a.a. 1-173), MW=19.7kD, expressed in an E.coli expression system.\n\nThe protein encoded by this gene belongs to a small class of prenylated protein tyrosine phosphatases (PTPs), which contains a PTP domain and a characteristic C-terminal prenylation motif. PTPs are cell signaling molecules that play regulatory roles in a variety of cellular processes. This tyrosine phosphatase is a nuclear protein, but may primarily associate with plasma membrane. The surface membrane association of this protein depends on its C-terminal prenylation. Overexpression of this gene in mammalian cells conferred a transformed phenotype, which implicated its role in the tumorigenesis. Studies in rat suggested that this gene may be an immediate-early gene in mitogen-stimulated cells.\n\nApplication: useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors.\n\nPurity: (same/more than)95% by SDS-PAGE.\n\nSpecific Activity: 0.45 U/ug. One unit will hydrolyze 1 pmol 6, 8-difluoro-4-methylumbelliferyl phosphate (DiFMUP) per minute at pH 6.3 and 30 degrees C\n\nAssay buffer: 50 mM Bis-Tris, pH 6.3, 2 mM EDTA, 2 mM DTT, 100 mM DiFMUP.\n\nStability: 6 months at

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SPECIFICATIONS

Catalog Number

P9003-68

Size

50ug

Reactivities

Hum

Form

25 Mm Tris-HCl, pH 8.0, 75 mM sodium chloride, 0.05% Tween-20, 50% glycerol, 2mM EDTA, 3mM DTT.

References

1. Q. Zeng et al., Cancer Res. 2003, 63: 2716-2722.\n2. J. Wang et al., J. Biol. Chem 2002, 277: 46659-46668.

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