PRL-3, aa2-173, Recombinant, Human, Protein Tyrosine Phosphatase, catalytic domain (Phosphatase of regenerating liver-3)

PRL-3 Protein tyrosine kinase (PTKs) and protein tyrosine phosphatase (PTPs) play a fundamental role in regulating diverse cellular processes. One primary growth response gene, ?rst identi?ed in regenerating liver, encodes a novel 20kD nuclear PTP known as PRL-1 (phosphatase of regenerating liver). Other than the signature sequence for PTPs, PRL-1 is not homologous to either the dual speci?city PTPs (cdc25 and MKP-1) or monospeci?c PTPs. Database searches using the PRL-1 sequence led to the identi?cation of mouse PRL-2 and PRL-3, which exhibit 87% and 76% identity to mouse PRL-1 in their amino acid Sequence. All PRL proteins bear signi?cant sequence homology to cdc14p and recently identi?ed tumor suppressor PTEN/MMAC1. Northern blot analysis revealed that PRL-2 is preferentially expressed in skeletal muscle, while PRL-3 is preferentially expressed in smooth muscle, skeletal muscle and heart muscle. Both PRL-2 and PRL-3 are expressed at lower levels in other tissues. Human PRL-3 exhibits more than 95% identity to mouse PRL-3. It was identi?ed in a cDNA library from an explanted human heart obtained from a patient with idiopathic cardiomyopathy It has been reported that PRL-3 plays a role in the modulation of intracellular calcium transients induced by angiotensin-II. It has also been shown that the PRL-3 gene may be related to colorectal cancer metastasis.\n\nCatalytic domain, (a.a. 1-173), MW=19.4 kD, expressed in an E.coli expression system.\n\nSpecific Activity: \n0.9 U/ug. One unit will hydrolyze 1 pmol 6, 8-difluoro-4-methyl umbelliferyl phosphate (DiFMUP) per minute at pH 6.3 and 30 degrees C.\n\nApplications:\nSuitable for the study of enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors.\n\nAssay Buffer: \n50 mM Bis-Tris, pH 6.3, 2 mM EDTA, 2 mM DTT, 100 mM DiFMUP.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -70 degrees C. Aliquots are stable for at least 6 months at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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