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Profilin 2, Recombinant, Human (Profilin-2, Profilin II, Profilin-II, PFN2, PFN-2, D3S1319E, PFL)

Cat no: P9005-89W

Profilin 2, Recombinant, Human (Profilin-2, Profilin II, Profilin-II, PFN2, PFN-2, D3S1319E, PFL)

PFN2 is a ubiquitous actin monomer-binding protein belonging to the profilin family. This protein is ubiquitously expressed with highest expression in kidney, brain and skeletal muscle. Like other members of the profilin family, PFN2 functions as an actin monomer-binding protein that influences the structure of the cytoskeleton by regulating actin polymerization in response to extracellular signals. At high concentrations, it prevents the polymerization of actin whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. Recombinant PFN2 protein, fused to His-tag at N-terminus, was expressed in E. coli.\n\nSource: \nRecombinant corresponding to aa1-140 of human PFN2 6x His tagged expressed in E. coli.\n\nAA Sequence:\nMGSSHHHHHH SSGLVPRGSH MAGWQSYVDN LMCDGCCQEA AIVGYCDAKY VWAATAGGVF QSITPIEIDM IVGKDREGFF TNGLALGAKK CSVIRDSLYV DGDCTMDIRT KSQGGEPTYN VAVGRAGRVL VFVMGKEGVH GGGLNKKAYS MAKYLRDSGF\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

P9005-89W

Size

50ug

Applications

WB

Form

Supplied as a liquid in 20mM Tris HCl, pH 8.0, 1mM DTT, 10% glycerol.

Purity

~ 95% (SDS-PAGE). Purified by using conventional chromatography techniques.

References

1. Gieslmann R., et al. (1995) Eur J Bilchem. 229(3):621-8. 2.Reinhard M., et al. (1995) EMBO J. 14(8):1583-9.

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