Progesterone, Human, BioAssay(TM) ELISA Kit

The United States Biological Progesterone, Human, BioAssay(TM) ELISA Kit is for the quantitative determination of Progesterone concentration in human serum or plasma. \n\nProgesterone is a C21 steroid which is synthesized from both tissue and circulating cholesterol. Cholesterol is transformed to pregnenolone which is then converted via a combined dehydrogenase and isomerase to progesterone. The principle production sites are the adrenals and ovaries and the placenta during pregnancy. The majority of this steroid is metabolized in the liver to pregnanediol and conjugated as a glucuronide prior to excretion by the kidneys. Progesterone exhibits a wide variety of end organ effects. The primary role of progesterone is exhibited by the reproductive organs. In males, progesterone is a necessary intermediate for the production of corticosteroids and androgens. In females, progesterone remains relatively constant throughout the follicular phase of the menstrual cycle. The concentration then increases rapidly following ovulation and remains elevated for 4-6 days and decreases to the initial level 24 hours before the onset of menstruation. In pregnancy, placental progesterone production rises steadily to levels of 10 to 20 times those of the luteal phase peak. Progesterone measurements are thus performed to determine ovulation as well as to characterize luteal phase defects. Monitoring of progesterone therapy and early stage pregnancy evaluations comprise the remaining uses of progesterone assays.\n\nKit Components: \n1. Microtiter Plate: 1x96 wells\n2. Progesterone Reference Standards: 6X500ul (0, 0.5, 3, 10, 25, 50ng/ml)\n3. Rabbit Anti-Progesterone Reagent: 1X7ml \n4. Progesterone (HRP), 11x: 1X1.3ml \n5. Progesterone (HRP) Diluent: 1X13ml \n6. Control 1, liquid: 1X500ul \n7. Control 2, liquid: 1X500ul \n8. Tetramethylbenzidine (TMB): 1x11ml\n9. Stop Solution: 1X11ml (1N HCl)\n\nStorage and Stability:\nStore all components at 4 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

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SPECIFICATIONS

Catalog Number

P9006-08H

Size

1Kit

Applications

ELISA

Reactivities

Hum

References

1. Radwanska, E., et al., Fertility and Sterility, 1978; 30: 398-402. 2. Autrere,M.B., and Benson, H., Progesterone: An overview and recent advances, J. Par. Sci., 1976; 65: 783-800. 3. March, C.M., Goebelsmann, U., Nakamura, R.M., and Mishell, D.R. Jr., Roles of estradiol and progesterone in eliciting the midcycle luteinizing hormone and follicle-stimulating hormone surges, J. Clin. Endo. Metab., 1979; 49: 507-513. 4. Ross, G.T., et al., Textbook of Endocrinology. Saunders Company, Philadelphia; 1981: 355-411. 5. Chattoraj, S.C., Endocrine function. In: Tietz, N.W., ed., Fundamentals of Clinical Chemistry. Saunders Company, Philadelphia; 1976: 699-823. 6. Shepard, M.K., and Senturia, Y.D., Comparison of serum progesterone and endometrial biopsy for confirmation of ovulation and evaluation of luteal function. Fertility and Sterility, 1977; 28: 541-548. 7. Johansson, E.D.B., and Jonasson, L.-E., Progesterone levels in amniotic fluid and plasma from women: I. Levels during normal pregnancy. Acta Obstet. Gynec. Scand., 1971; 50: 339-343. 8. USA Center for Disease Control/National Institute of Health Manual,