Prolactin Receptor (PRLR, PRL-R, PRL Receptor)

Prolactin (PRL) is a neuroendocrine hormone that is closely related to growth hormones and more distantly related to the peptide hormones of the interleukin family. The endocrine effects of PRL on human breast tissues include the regulation of growth and differentiation of ductal epithelium, proliferation and differentiation of lobular units, and initiation and maintenance of lactation. PRL and growth hormone receptors are members of the cytokine receptor superfamily. Both the PRLr and growth hormone receptors are single-chain transmembrane proteins composed of extracellular, transmembrane, and intracellular domains. Prolactin modulates immune system function by stimulating the proliferation and survival of cells through its receptor, present on T and B lymphocytes and macrophages. The function of PRL is mediated by a variety of signaling cascades as well as by the wide variety of PRLr isoforms observed in nature. A long form and several other isoforms are expressed in human tissues. The nucleotide sequence of the novel intermediate isoform of PRLr is identical to the long isoform, except for a 573-base pair deletion occurring at a consensus splice site, resulting in a frameshift and truncated intracytoplasmic domain. The long isoform of PRLr is N-glycosylated. As the extracellular domain of the intermediate isoform is identical to that of the long isoform, glycosylation patterns are assumed to be similar. Using immunohistochemical analysis, PRLr expression has been observed in 95% of both normal and malignant breast epithelium; no correlation between PRL expression and ER-PR status has been established.\n\nApplications:\nSutiable for use in Immunohistochemistry, Immunoprecipitation and Western Blot. Other applications have not been tested.\n\nRecommended Dilutions:\nImmunohistochemistry (Paraffin): 5-10ug/ml\nImmunoprecipitation: 5-10ug/ml\nWestern Blot: 1-3ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

P9009-59

Size

100ug

Applications

IHC, IP, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

P Type

Mab

Purity

Purified by Protein A chromatography

Isotype

IgG2b,k

References

1. Kline JB, et al. J Biol Chem 274(50):35461-35468, 1999. 2. Kline JB, et al. J Biol Chem 276(27):24760-24766, 2001. 3. Pellegrini I, et al. Mol Endocrinol 6(7):1023-1031, 1992. 4. Clevenger CV, et al. Am J Pathol 146(3):695-705, 1995. 5. Clevenger CV, et al. J Endocrinol 157(2):187-197, 1998. 6. Dorato A, et al. Endocrinology 131(4):1734-1742, 1992. 7. Reynolds C, et al. Endocrinology 138(12):5555-5560, 1997.

Additional Info

Recognizes the long form of the human prolactin receptor (hPRLr) protein at ~85-90kD. May also identify the human prolactin receptor intermediate and delta S1 isoforms by Western Blot. On Immunoprecipitation, this antibody strongly recognizes only the long form of hPRLr. Reactivity is confirmed with human T-47D breast carcinoma cells.