Prolactin, Recombinant, Human (PRL)

Source:\nRecombinant corresponding to 200aa of human Prolactin expressed in E.coli is a single, non-glycosylated polypeptide chain.\n\nMolecular Weight:\n~23kD\n\nBiological Activity:\nThe ED50 as determined by the dose-dependent stimulation of the proliferation of rat lymphoma, Nb2-11 was found to be <0.065nug/ml\n\nDimers and Aggregates:\n(same/less than)1% (SDS-PAGE)\n\nProtein Content:\nProtein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280nm. 2. Analysis by RP-HPLC, using a calibrated solution of Prolactin as a Reference Standard.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O, HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

P9009-03G

Size

50ug

Form

Supplied as a lyophilized powder. No preservative added. Reconstitute with sterile dH2O, 0.1% HSA or BSA to (same/more than)0.1mg/ml.

Purity

~95% (RP-HPLC, Anion-exchange FPLC, SDS-PAGE) Endotoxin: (same/less than)0.1ng/ug (IEU/ug)

References

1. Solution structure of human prolactin. J Mol Biol 2005 Aug 26;35 1(4):810-23. 2. The antiangiogenic factor, 16-kDa human prolactin, induces endothelial cell cycle arrest by acting at both the G0-G1 and the G2-M phases. Mol Endocrinol 2005 Jul;19(7):1932-42. 3. Antitumor effects of recombinant human prolactin in human adenocarcinoma-bearing SCID mice with human NK cell xenograft. Int Immunopharmacol 2005 Feb;5(2):417-25. 4. EGF stimulates Pit-1 independent transcription of the human prolactin pituitary promoter in human breast cancer SK-BR-3 cells through its proximal AP-1 response element. Mol Cell Endocrinol 2005 Jan 14;229(1-2):127-39. 5. Mechanism for ordered receptor binding by human prolactin. Biochemistry 2004 Nov 2;43(43):13755-65. 6. Multiple new isoforms of the human prolactin receptor gene. Adv Exp Med Biol 2004;554:495-9.

Alternative Names

OTTHUMP00000017727