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Prostate Cell, Immortalized Normal, Serum Free Media (BRFF P4-8F)

Cat no: P9054-01

Prostate Cell, Immortalized Normal, Serum Free Media (BRFF P4-8F)

BRFF-P4-8F(TM) was designed to grow the immortalized normal prostatic cell line 267-B1. It also supports the growth of certain established human prostatic cancer cell lines such as PC-3. For optimum cell attachment, use FNC Coating Mix(R).\nReady-to-use with all necessary growth factors. Antibiotic-free.\n\nStorage: \nStore at -70 degrees C. Supplied frozen.\n\nStability at 4 degrees C: \n4-6 weeks\n\npH: \n7.2-7.5 (5% CO2 atmosphere is recommended for culture.)\n\nOsmolality: \n270-300mOsM\n\nSterility Test:\nSpecification: Detection of microbial growth.\nTest Sample: 0.1ml into each of the following media (in triplicate)\nIncubation Conditions: 37 degrees C/48 hrs\n\nFunctional Test:\nSpecification: Growth of cell line 276-B1 to confluence. One vial containing 1-5x10e6 cells is quick thawed, washed with 10ml of medium, suspended in 10ml of medium and applied to a T-25 flask pre-coated with FNC Coating Mix(R). The culture is incubated at 37 degrees C in a 5.0% CO2 incubator.

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SPECIFICATIONS

Catalog Number

P9054-01

Size

500ml

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