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Protein Phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alpha isoform  (PPP2R1A, MGC786, protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alpha isoform, PP2A, subunit A, R1-alpha isoform, PP2A, subunit A, PR65-alpha isoform, m

Cat no: P9107-14

Protein Phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alpha isoform (PPP2R1A, MGC786, protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alpha isoform, PP2A, subunit A, R1-alpha isoform, PP2A, subunit A, PR65-alpha isoform, m

Alternate Name: PPP2R1A, MGC786, protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alpha isoform, PP2A, subunit A, R1-alpha isoform, PP2A, subunit A, PR65-alpha isoform, medium tumor antigen-associated 61 KDA protein, Serine/threonine protein phosphatase 2A, 65 KDA regulatory subunit A, alpha isoform\n\nDomain Information: \n\nGene Ontology Terms: RNA splicing; antigen binding; binding; ceramide metabolism; cytosol; inactivation of MAPK; induction of apoptosis; membrane; microtubule cytoskeleton; mitochondrion; negative regulation of cell growth; negative regulation of tyrosine phosphorylation of Stat3 protein; nucleus; phosphoprotein phosphatase activity; protein amino acid dephosphorylation; protein binding; protein complex assembly; protein heterodimerization activity; protein phosphatase type 2A activity; protein phosphatase type 2A complex; protein phosphatase type 2A regulator activity; regulation of DNA replication; regulation of Wnt receptor signaling pathway; regulation of cell adhesion; regulation of cell cycle; regulation of cell differentiation; regulation of growth; regulation of transcription; regulation of translation; response to organic substance; second-messenger-mediated signaling; soluble fraction\n\nApplication(s):\nPeptide ELISA: antibody detection limit dilution 1:8,000. Western Blot: Approx 60kD band observed in Human Thymus and Lymph Node lysates (calculated MW of 65.3kD according to NP_055040.2). Recommended concentration: 0.03-0.1ug/ml.\n\n0.03ug/ml staining of Human Lymph Node lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\nPeptide Blocking:\nCorresponding peptide is available for Peptide Blocking studies. See P9107-14-P. Antibody

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SPECIFICATIONS

Catalog Number

P9107-14

Size

100ug

Applications

ELISA, WB

Hosts

Goat

Reactivities

Hum, Mouse, Rat, Can

Form

Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

Hong Y, Sarge KD. , Regulation of protein phosphatase 2A activity by heat shock transcription factor 2., J Biol Chem. 1999 May 7;274(19):12967-70.

Additional Info

Recognizes Human, Mouse, Rat, canine PP2A/ PPP2R1A

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Applications

ELISA

Reactivities

Hum

More info

Applications

IF

Hosts

Mouse

More info

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info

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