PTEN Tumor Suppressor Protein, phosphorylated (Ser380, Thr382, Ser385) (MMAC1, Phosphatase and Tensin, TEP1)

PTEN (Phosphatase and tensin analog) is a ~58-60kD lipid phosphatase and tumor suppressor protein that is frequently mutated in tumor cells. It plays a key role in cell migration, survival and apoptosis by negatively regulating phosphoproteins in the Akt/PI3Kinase pathway. The noncatalytic regulatory carboxyl terminus of PTEN contains multiple phosphorylation sites including two CK2 sites (serines 370 and 385) and MAGI-2 sites (threonines 382 and 383). Phosphorylation of serine 370 and 385 primes PTEN for additional phosphorylation of nearby residues while phosphorylation of serine 380 and threonines 382 and 385 plays a critical role in regulating PTEN stability. \n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \n3T3-L1 adipocytes; Hek293 cells overexpressing PTEN\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

P9182-15

Size

10 Blots

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide, 50% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

1. Raftopoulou, M., et al. (2004) Regulation of cell migration by the C2 domain of the tumo suppressor PTEN. Science 303(5661):1179-1181. 2. Mourani, P.M., et al. (2004) Unique, highly proliferative growth phenotype expressed by embryonic and neointimal smooth muscle cells is driven by constitutive Akt, mTOR, and p70S6K signaling and is actively repressed by PTEN. Circulation 109(10):1299-1306. 3. Wan, X. and L.J. Helman (2003) Levels of PTEN protein modulate Akt phosphorylation on serine 473, but not on threonine 308, in IGF-II-overexpressing rhabdomyosarcomas cells. Oncogene 22(50):8205-8211. 4. Miller, S.J., et al. (2002) Direct identification of PTEN phosphorylation sites. FEBS Lett. 528 (1-3):145-153. 5. Birle, D., et al. (2002) Negative feedback regulation of the tumor suppressor PTEN by phosphoinositide-induced serine phosphorylation. J. Immunol. 169(1):286-291. 6. Mohiuddin, I., et al. (2002) Phosphatase and tensin analog gene overexpression engenders cellular death in human malignant mesothelioma cells via inhibition of AKT phosphorylation. Ann. Surg. Oncol. 9(3):310-316. 7. Persad, S., et al. (2001) Tumor suppressor PTEN inhibits nuclear accumulation of beta-catenin and T cell/lymphoid enhancer factor 1-mediated transcriptional activation. J. Cell. Biol. 153(6):1161-1174. 8. Torres, J. and R. Pulido (2001) The tumor suppressor PTEN is phosphorylated by the protein kinase CK2 at its C terminus. Implications for PTEN stability to proteasome-mediated degradation. J. Biol. Chem. 276(2):993-998.

Additional Info

Recognizes human PTEN when phosphorylated at Ser380, Thr382 and Ser385. Species Crossreactivity: Mouse PTEN. Species sequence homology: Rat (100%).