RAD9A pAb

DNA damage or incomplete replication of DNA results in the inhibition of cell cycle progression at the G1 to S or G2 to M phase checkpoints by conserved regulatory mechanisms. Chk1, Rad9 and Hus1 are involved in the signal transduction cascade that regulates cell cycle arrest at the G2 checkpoint. Chk1 functions as an essential component in the G2 phase DNA damage checkpoint, as it phosphorylates Cdc25C in response to DNA damage and thereby inhibits mitosis. Two related mammalian proteins, Hus1 and Rad9, share conserved sequence identity and function to the yeast homologs of the same names. In vivo, Rad9 is highly phosphorylated and directly associates with two other checkpoint control proteins, Rad1 and Hus1. Additionally, Rad9 associates with anti-apoptotic Bcl-2 family proteins Bcl-2 and Bcl-xL, but not with the pro-apoptotic Bax and Bad proteins. Overexpression of Rad9 induces apoptosis and indicates that Rad9 may have an additional role in regulating apoptosis after DNA damage.

Prices direct from Bioworld Technology, Inc.

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

BS6718

Size

100ug/100ul

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

liquid

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Antigen

Recombinant full length Human RAD9A.

Species

RAD9A pAb detects endogenous levels of RAD9A protein.

Dilutions

WB: 1:500~1:2000\nIHC: 1:50~1:200

Swiss Prot

Q99638

Storage Temp

Store at 4 degrees C short term. Aliquot and store at -20 degrees C long term. Avoid freeze-thaw cycles.

Concentration

1ug/ul

Storage Buffer

1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.

Additional Info

For research use only, not for use in diagnostic procedure.

Molecular Weight

~ 45 kDa

Alternative Names

Cell cycle checkpoint control protein; Cell cycle checkpoint control protein RAD9A; DNA repair exonuclease rad9 homolog A; hRAD 9; hRAD9; Rad 9; RAD 9A; RAD9 (S pombe) homolog; RAD9 homolog A; RAD9 homolog; RAD9A; RAD9A;