RANBP2, CT (Ran-binding Protein 2, RanBP2, Nuclear Pore Complex Protein Nup358, Nucleoporin Nup358, 358kD Nucleoporin, P270, E3 SUMO-protein Ligase RanBP2, NUP358) (APC)

RAN is a small GTP-binding protein of the RAS superfamily that is associated with the nuclear membrane and is thought to control a variety of cellular functions through its interactions with other proteins. This gene encodes a very large RAN-binding protein that immunolocalizes to the nuclear pore complex. The protein is a giant scaffold and mosaic cyclophilin-related nucleoporin implicated in the Ran-GTPase cycle. The encoded protein directly interacts with the E2 enzyme UBC9 and strongly enhances SUMO1 transfer from UBC9 to the SUMO1 target SP100. These findings place sumoylation at the cytoplasmic filaments of the nuclear pore complex and suggest that, for some substrates, modification and nuclear import are linked events. This gene is partially duplicated in a gene cluster that lies in a hot spot for recombination on chromosome 2q.\n\nApplications: \nSuitable for use in ELISA and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:1,000\nImmunohistochemistry: 1:50-1:100\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze APC conjugates. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

R1069-11A-APC

Size

200ul

Applications

ELISA, IHC

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2. Contains ~0.09% sodium azide. Conjugated to Allophycocyanin (APC).

P Type

Pab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG

References

1.Salina, D., et al., J. Cell Biol. 162(6):991-1001 (2003). 2.Kirsh, O., et al., EMBO J. 21(11):2682-2691 (2002). 3.Mavlyutov, T.A., et al., Traffic 3(9):630-640 (2002). 4.Miyauchi, Y., et al., J. Biol. Chem. 277(51):50131-50136 (2002). 5.Saitoh, H., et al., J. Biol. Chem. 277(7):4755-4763 (2002).

Additional Info

Recognizes human RANBP2.