RANTES (Regulated on Activation Normal T-cell Expressed and Secreted, Regulated Upon Activation Normally T Expressed and Presumably Secreted, Beta Chemokine RANTES Precursor, CCL5, Chemokine (C-C Motif) Ligand 5, Chemokine CC Motif Ligand 5, D17S136E, MGC

CCL5, also known as RANTES (Regulated upon Activation, Normal T cell Expressed and presumably Secreted), is an 8kD b-chemokine that plays a primary role in the inflammatory immune response by means of its ability to attract and activate leukocytes (1-3). Human and mouse RANTES exhibit cross-species activity on human and mouse cells (4). Mature canine CCL5 shares 74-84aa seqeuence identity with cotton rat, feline, human, mouse, and rat CCL5. CCL5 is secreted by many cell types at inflammatory sites, and it exerts a wide range of activities through the receptors CCR1, CCR3, CCR4, and CCR5 (5, 6). Inflammatory responses can be impaired by the sequestration of CCL5 by the cytomegalovirus protein US28 (7). In humans, CCR5 binding to CCL5 inhibits the infectivity of R5 (M-tropic) but not X4 (T-tropic) strains of HIV-1 (8). The two N-terminal residues of CCL5 can be removed by CD26/DPPIV, generating a protein that functions as a chemotaxis inhibitor and more effectively blocks M-tropic HIV-1 infection of monocytes (9). Oligomerization of CCL5 on glycosaminoglycans is required for CCR1-mediated leukocyte adhesion and activation as well as CCL5

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SPECIFICATIONS

Catalog Number

R1100-15E

Size

100ug

Hosts

Mouse

Reactivities

Can

Form

Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml.

P Type

Mab

Purity

Purified by Protein A/G affinity chromatography.

Isotype

IgG1

References

1. Schall, T.J. et al. (1990) Nature 347:669. 2. Bacon, K.B. et al. (1995) Science 269:1727. 3. Fischer, F.R. et al. (2001) J. Immunol. 167:1637. 4. Schall, T.J. et al. (1992) Eur. J. Immunol. 22:1477. 5. Appay, V. and S.L. Rowland-Jones (2001) Trends Immunol. 22:83. 6. Levy, J.A. (2009) J. Immunol. 182:3945. 7. Randolph-Habecker, J.R. et al. (2002) Cytokine 19:37. 8. DeVico, A.L. and Gallo, R.C. (2004) Nat. Rev. Microbiol. 2:401. 9. Proost, P. et al. (1998) J. Biol. Chem. 273:7222. 10. Appay, V. et al. (1999) J. Biol. Chem. 274:27505. 11. Proudfoot, A.E.I. et al. (2003) Proc. Natl. Acad. Sci. 100:1885. Rev. 2/14/2011 Page 1 of 2 16. Soria, G. and A. Ben-Baruch (2008) Cancer Lett. 267:271.

Additional Info

Recognizes canine RANTES. Does not react with recombinant RANTES from cat, cotton rat, mouse, human, recombinant mouse CCL7, rmCCL17, recombinant human CCL18, rhCCL17.