Soluble Fas Receptor, Recombinant, Human (ALPS1A, Apo-1 Antigen, Apoptosis-mediating Surface Antigen FAS, APT1, CD95, FAS1, FASLG Receptor, FASTM, Tumor Necrosis Factor Receptor Superfamily Member 6, TNFRSF6)

Fas and Fas Ligand (FasL) belong to the TNF superfamily and are type I and type II transmembrane proteins, respectively. Binding of FasL to Fas triggers apoptosis in Fas-bearing cells. The mechanism of apoptosis involves recruitment of pro-caspase 8 through an adaptor molecule called FADD followed by processing of the pro-enzyme to active forms. These active caspases then cleave various cellular substrates leading to the eventual cell death. sFasR is capable of inhibiting FasL-induced apoptosis by acting as a decoy receptor that serves as a sink for FasL. The full length Fas (receptor) is a 319aa type I transmembrane protein, which contains a 157aa extracellular domain, a 17aa transmembrane domain, and 145aa cytoplasmic domain. Recombinant human soluble Fas (sFas Receptor) is a 157aa polypeptide,17.6kD, corresponding to the TNFR homologous cysteine rich extracellular domain Fas.\n\nSource:\nRecombinant protein corresponding to human Soluble Fas Receptor, expressed in E.coli.\n\nBiological Activity:\nDetermined by its ability to inhibit the cytotoxicity of Jurkat cells. The ED50 was found to be between 10-15ug/ml in the presence of 2ng/ml of hFasL.\n\nEndotoxin:\n<0.1ng/ug (1EU/ug)\n\nAA Sequence:\nMRLSSKSVNA QVTDINSKGL ELRKTVTTVE TQNLEGLHHD GQFCHKPCPP GERKARDCTV NGDEPDCVPC QEGKEYTDKA HFSSKCRRCR LCDEGHGLEV EINCTRTQNT KCRCKPNFFC NSTVCEHCDP CTKCEHGIIK ECTLTSNTKC KEEGSRS\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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