Fas and Fas Ligand (FasL) belong to the TNF superfamily and are type I and type II transmembrane proteins, respectively. Binding of FasL to Fas triggers apoptosis in Fas-bearing cells. The mechanism of apoptosis involves recruitment of pro-caspase 8 through an adaptor molecule called FADD followed by processing of the pro-enzyme to active forms. These active caspases then cleave various cellular substrates leading to the eventual cell death. sFasR is capable of inhibiting FasL-induced apoptosis by acting as a decoy receptor that serves as a sink for FasL. The full length Fas (receptor) is a 319aa type I transmembrane protein, which contains a 157aa extracellular domain, a 17aa transmembrane domain, and 145aa cytoplasmic domain. Recombinant human soluble Fas (sFas Receptor) is a 157aa polypeptide,17.6kD, corresponding to the TNFR homologous cysteine rich extracellular domain Fas.
Source:
Recombinant protein corresponding to human Soluble Fas Receptor, expressed in E.coli.
Biological Activity:
Determined by its ability to inhibit the cytotoxicity of Jurkat cells. The ED50 was found to be between 10-15ug/ml in the presence of 2ng/ml of hFasL.
Endotoxin:
<0.1ng/ug (1EU/ug)
AA Sequence:
MRLSSKSVNA QVTDINSKGL ELRKTVTTVE TQNLEGLHHD GQFCHKPCPP GERKARDCTV NGDEPDCVPC QEGKEYTDKA HFSSKCRRCR LCDEGHGLEV EINCTRTQNT KCRCKPNFFC NSTVCEHCDP CTKCEHGIIK ECTLTSNTKC KEEGSRS
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.