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STAT 6 (STAT6B, STAT6C, D12S1644, IL-4-STAT, interleukin 4 induced STAT, Signal Transducer and Activator of Transcription 6)

Cat no: S7972-26E

STAT 6 (STAT6B, STAT6C, D12S1644, IL-4-STAT, interleukin 4 induced STAT, Signal Transducer and Activator of Transcription 6)

The STAT proteins serve as both cytoplasmic signal transducers and nuclear activators of transcription. STATs are mediators involved in cytokine signalling. In response to a specific cytokine signal, STAT proteins are phosphorylated on conserved tyrosine residues. Phosphorylated STAT proteins dimerize via their SH2 domains and move to the nucleus. The STAT dimers bind to specific DNA elements resulting in transcriptional regulation of downstream target genes. STAT6 is activated primarily by IL-4 and IL-13. Upon activation, STAT6 is phosphorylated at tyrosine 641 by Janus Kinase (JAK). Phosphorylated STAT6 forms head-to-tail heterodimers and translocates to the nucleus where it paricipates in transcriptional control.\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution: \nELISA: 0.05ug/ml\nWestern Blot: 0.1ug/ml for HRPO/ECL detection. 1ug/ml for AP/BCIP/NBT (MTT) detection.\nOptimal dilution to be determined by researcher.\n\nIncluded Positive Control:\nH2030-03H: HepG2 Cell Lysate, Untreated\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S7972-26E

Size

50ug

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, 0.09% sodium azide, PEG, sucrose.

P Type

Mab

Purity

Purified by subsequent thiophilic absorption and size exclusion chromatography.

Isotype

IgG1

Additional Info

Recognizes human STAT6. Detects a band of ~97kD by Western Blot. Species Crossreactivity: mouse.

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