TNFRSF9/4-1BB BioAssay(TM) ELISA Kit, Human

Sandwich ELISA quantitative detection kit for Human TNFRSF9/4-1BB in cell culture supernates, serum and plasma (heparin, EDTA).\n\nCD137 is a member of the tumor necrosis factor (TNF) receptor family. Its alternative names are tumor necrosis factor receptor superfamily member 9 (TNFRSF9), 4-1BB and induced by lymphocyte activation (ILA). CD137 gene was localized to chromosome 1p36.Members of this receptor family and their structurally related ligands are important regulators of a wide variety of physiological processes and play an especially important role in the regulation of immune responses. CD137 can be expressed by activated T cells, but to a larger extent on CD8 than on CD4 T cells. The best characterized activity of CD137 is its costimulatory activity for activated T cells. Crosslinking of CD137 enhances T cell proliferation, IL-2 secretion survival and cytolytic activity. Further, it can enhance immune activity to eliminate tumors in mice.\n\nAssay Range:\n31.2pg/ml-2000pg/ml\n\nSensitivity:\n< 10 pg/ml\n\nCrossreactivity:\nThere is no detectable cross-reactivity with other relevant proteins.\n\nUniprot ID:\nQ07011\n\nEntrez Gene ID:\n3604\n\nKit Components:\n96-well plate pre-coated with anti-TNFRSF9/4-1BB, Human\nLyophilized recombinant TNFRSF9/4-1BB, Human standard, 10ng/tube, 2Ea\nUSB Cat No Kit Component\n143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)\n143697 Sample diluent buffer, 30ml\n143698 Antibody diluent buffer, 12ml\n143699 ABC diluent buffer, 12ml\n143700 TMB color developing agent, 10ml\n143701 TMB stop solution, 10ml\n\nStorage and Handling:\nStore at 4 degrees C for 6 months, at -20 degrees C for 12 months. Avoid multiple freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

144076

Size

96Tests

References

1. Jang, I K; Lee Z H, Kim Y J, Kim S H, Kwon B S (Jan. 1998). \"Human 4-1BB (CD137) signals are mediated by TRAF2 and activate nuclear factor-kappa B\". Biochem. Biophys. Res. Commun. (UNITED STATES) 242 (3): 613