TRAF2, or Tumor Necrosis factor (TNF) Receptor-Associated Factor 2, is an adapter protein and signal transducer that links members of the tumor necrosis factor receptor family to different signaling pathways by association with the receptor cytoplasmic domain and kinases. Association to the receptor is also mediated by the interaction with TRADD. TRAF2 mediates activation of NF-kappa-B and MAPK8/JNK and is involved in apoptosis. TRAF2 forms a heterodimeric complex with TRAF1, which then recruits the inhibitor-of-apoptosis proteins (IAPs),
apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2 for the inhibition of caspase
activation. In this way it functions as a mediator of the anti-apoptotic signals from TNF receptors. BIRC2/c- IAP1, an apoptosis inhibitor possessing ubiquitin ligase activity, can unbiquitinate and induce the degradation of this protein, and thus potentiate TNF-induced apoptosis. TRAF2 may be involved in IL-15 signaling. Multiple alternatively spliced transcript variants exist, but the biological validity of only one transcript has been determined.
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
ELISA: 1:60,000-1:250,000
Western Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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