Trefoil Factor 3, Intestinal (TFF3) BioAssay(TM) ELISA Kit (Mouse) is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of mouse TFF3 in serum, plasma and other biological fluids.
Detection Range:
61.73-5,000pg/ml
Sensitivity:
29.14pg/ml
Intra-Assay:
CV<10%
Inter-Assay:
CV<12%
Kit Components:
*028606A: Microtiter Strip, 1x96 wells, Pre-coated, ready to use
*028606B: Standard, 2x1vial�
028606C: Standard Diluent, 1x20ml
*028606D: Detection Reagent A, 1x120ul�
*028606E: Detection Reagent B, 1x120ul�
028606F: Assay Diluent A, 1x12ml
028606G: Assay Diluent B, 1x12ml
028606H: TMB Substrate, 1x9ml
028606K: Stop Solution, 1x6ml
028606L: Wash Buffer, 30X, 1x20ml
Storage and Stability:
Store *028606A, *028606B, *028606D and *028606E at -20 degrees C. Store all the other components at 4 degrees C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
Assay Summary:
1. Prepare all reagents, samples and standards.
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37 degrees C.
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37 degrees C.
4. Aspirate and wash 3 times.
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37 degrees C.
6. Aspirate and wash 5 times.
7. Add 90ul TMB Substrate. Incubate 15-25 minutes at 37 degrees C.
8. Add 50ul Stop Solution. Read at 450nm immediately.
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