Trefoil Factor 3, Intestinal (TFF3) BioAssay(TM) ELISA Kit (Mouse)

Trefoil Factor 3, Intestinal (TFF3) BioAssay(TM) ELISA Kit (Mouse) is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of mouse TFF3 in serum, plasma and other biological fluids.\n\nDetection Range:\n61.73-5,000pg/ml\n\nSensitivity:\n29.14pg/ml\n\nIntra-Assay: \nCV<10%\n\nInter-Assay: \nCV<12%\n\nKit Components:\n*028606A: Microtiter Strip, 1x96 wells, Pre-coated, ready to use\n*028606B: Standard, 2x1vialпїЅ\n028606C: Standard Diluent, 1x20ml\n*028606D: Detection Reagent A, 1x120ulпїЅ\n*028606E: Detection Reagent B, 1x120ulпїЅ\n028606F: Assay Diluent A, 1x12ml\n028606G: Assay Diluent B, 1x12ml\n028606H: TMB Substrate, 1x9ml\n028606K: Stop Solution, 1x6ml\n028606L: Wash Buffer, 30X, 1x20ml\n\nStorage and Stability:\nStore *028606A, *028606B, *028606D and *028606E at -20 degrees C. Store all the other components at 4 degrees C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.\n\nAssay Summary:\n1. Prepare all reagents, samples and standards.\n2. Add 100ul standard or sample to each well. Incubate 2 hours at 37 degrees C.\n3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37 degrees C.\n4. Aspirate and wash 3 times.\n5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37 degrees C.\n6. Aspirate and wash 5 times.\n7. Add 90ul TMB Substrate. Incubate 15-25 minutes at 37 degrees C.\n8. Add 50ul Stop Solution. Read at 450nm immediately.

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