Quick Search
Products Trypsin, Bovine Pancreas, TPCK Treated
| Catalogue number: | T8674 |
| Size: | 100mg |
| Form: | Supplied as a lyophilized powder that has been treated with L-(tosylamido-2-phenyl) ethyl chloromethyl ketone (TPCK) to inhibit contaminating chymotryptic activity. |
| Purity: | Chromatographically purified |
| Alternative names: | EC=3.4.21.4 |
| References: | US Biological application reference: Gainey, M.R. et al., (2009) Health 2:32-37. 1. Abita, J., and Lazdunski, M.: On the Structural and Functional Role of Carboxylates in Chymotrypsinogen A: A Comparison with Chymotrypsin, Trypsinogen and Trypsin, Biochem. Biophys. Res. Comm., 35, 707 (1969). 2. Abuchowski, A., and Davis, F.: Preparation and Properties of Polyethylene Glycol-Trypsin Adducts, Biochim. Biophys. Acta, 578, 41 (1979). 3. Anson, M.: The Estimation of Pepsin, Trypsin, Papain and Cathepsin with Hemoglobin, J. Gen. Physiol., 22, 79 (1938). 4. Antonini, E., and Ascenzi, P.: The Mechanism of Trypsin Catalysis at Low pH. Proposal for a Structural Model, J. Biol. Chem., 256, 12449 (1981). 5. Arakawa, T., Hung, L., Mcginley, M., Rohde, M., and Narhi, L.: Induced Resistance of Trypsin to Sodium Dodecylsulfate upon Complex Formation with Trypsin Inhibitor, J. Protein Chem., 11, 171 (1992). 6. Arnon, R., and Neurath, H.: An Immunological Approach to the Study of Evolution of Trypsins, Proc. Natl. Acad. Sci., 64, 1323 (1969). 7. Ascenzi, P., Amiconi, G., Bolognesi, M., Menegatti, E., and Guarneri, M.: Trypsin Activation. Effect of the Ile-Val Dipeptide Concentration on Kazal Inhibitor Binding to Bovine Trypsinogen, Biochim. Biophys. Acta, 832, 1985 (1985). Ascenzi, P., Bolognesi, M., Guarneri, M., Menegatti, E., and Amiconi, G.: Benzamidine as a Spectroscopic Probe for the Primary Specificity Subsite of Trypsin-like Proteinases, Mol. Cell. Biochem., 64, 139 (1984). Ascenzi, P., Menegatti, E., Bortolotti, F., Guarneri, M., and Antonini, E.: Steady-state and Pre-steady-state Kinetics of the Trypsin-catalyzed Hydrolysis of a-CBZ-L-Lysine-p-nitrophenyl Ester, Biochim. Biophys. Acta, 658, 158 (1981). Asgeirsson, B., Fox, J., and Bjarnason, J.: Purification and Characterization of Trypsin from the Poikilotherm Gladus morhua, Eur. J. Biochem., 180, 85 (1989). Atassi, P.: Surface-simulation Synthesis of the Substrate-binding Site of an Enzyme. Demonstration with Trypsin, Biochem. J., 226, 477 (1985). Baines, N., Baird, J., and Elmore, D.: The Kinetics of Hydrolysis of Derivatives of Arginine, Homoarginine and Ornithine by Trypsin, Biochem. J., 90, 470 (1964). Balls, A.: Concerning Trypsinogen, Proc. Natl. Acad. Sci., 53, 392 (1965). Bar-Eli, A., and Katchalski, E.: Water-Insoluble Trypsin Derivative and its Use as a Trypsin Column, Nature, 188, 856 (1960). Bartholomew, J., Handley, C., and Lowther, D.: The Effects of Trypsin Treatment on Proteoglycan Biosynthesis of Bovine Articular Cartilage, Biochem. J., 227, 429 (1985). Beardslee, R., and Zahnley, J.: A Simple Preparation of [[beta]]-Trypsin Based on a Colorimetric Study of the Thermal Stabilities of [[alpha]] and [[beta]] Trypsin, Arch. Biochem. Biophys., 158, 806 (1973). Berezin, I., Kazanskaya, N., Klyosov, A., and Svedas, V.: The Mechanism of the [[alpha]]-Chymotrypsin and Trypsin-Catalyzed Hydrolysis of Amides. Evidence for the Participation of the Active Serine in the Amidase Activity of Trypsin, Eur. J. Biochem., 38, 529 (1973). Bergmann, M., and Fruton, J.: in Advances in Enzymology, 63 (1941). Berlin, I., and Neujahr, H.: Studies of Controlled Lysis of Washed Cell Suspensions of Lactobacillus fermenti and Preparation of Membrane-like Fragments by a Combined Trypsin-Lysozyme Treatment, Acta Chem. Scand., 22, 2972 (1969). Berliner, L., and Wong, S.: Evidence Against Two "pH-locked" Conformations of Phosphorylated Trypsin, J. Biol. Chem., 248, 1118 (1973). Berliner, L., and Wong, S.: Spin-Labeled Sulfonyl Fluorides as Active Site Probes of Protease Structure. I. Comparison of the Active Site Environments in [[alpha]]-Chymotrypsin and Trypsin, J. Biol. Chem., 249, 1668 (1974). Bier, M., and Nord, F.: Arch. Biochem. Biophys., 33, 320 (1951). Borovsky, D., and Schlein, Y.: Quantitative Determination of Trypsin-like and Chymotrypsin-like Enzymes in Insects, Arch. Insect Biochem. Physiol., 8, 249 (1988). Bricteux -Gr |
Get the Best Promotions
