UCP1 (Solute carrier family 25 member 7, Thermogenin)

UCP1 (Uncoupling Protein 1), also called THERMOGENIN or UCP, is an uncoupling protein found in the mitochondria of brown adipose tissue (BAT). Using in situ hybridization, Cassard et al. (1990) assigned the human UCP gene to 4q31. They found that the primary structure of UCP is similar to that of ADP/ATP translocator of skeletal muscle, the gene for which is also located on chromosome 4. The failure of UCP1 expressed in E. coli inclusion bodies to carry out fatty acid-dependent H+ transport activity inclusion bodies made Echtay et al. (2000) seek a native UCP cofactor. They identified coenzyme Q (CoQ, or ubiquinone) as such a cofactor. On addition of CoQ(10) to reconstituted UCP1 from inclusion bodies, fatty acid-dependent proton transport reached the same rate as with native UCP1.\n\nUniProt Number:\nP25874\n\nGene ID:\nUCP1\n\nApplications:\nSuitable for use in Western Blot.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Handling:\nStore at -20?C for one year. After reconstitution, store at 4?C for one month. Can also be aliquoted and stored frozen at -20?C for long term.\nAvoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

145345

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Cassard, A. M., Bouillaud, F., Mattei, M. G., Hentz, E., Raimbault, S., Thomas, M., Ricquier, D. Human uncoupling protein gene: structure, comparison with rat gene, and assignment to the long arm of chromosome 4. J. Cell. Biochem. 43: 255-264, 1990.\n2. Echtay, K. S., Winkler, E., Klingenberg, M. Coenzyme Q is an obligatory cofactor for uncoupling protein function. Nature 408: 609-613, 2000.\n

Additional Info

Recognizes human UCP1. No crossreactivity with other proteins.