The uncoupling protein UCP1 (formally designated UCP) is an integral membrane protein unique to brown adipose tissue mitochondria. UCP1 forms a dimmer that acts as a protein channel, which can uncouple oxidative phosphorylation by dissipating the electrochemical potential across the inner mitochondrial membrane. This process induces heat production in brown adipose tissue and is involved in regulation of body temperature and glucose metabolism. UCP3 is highly muscle specific and is possibly involved in the uncoupling of oxidative phosphorylation in skeletal muscle.
Applications:
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution:
Western Blot: 1-2ug/ml for 2h at RT
Optimal dilutions to be determined by the researcher.
Positive Control:
Skeletal muscle
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.