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Uncoupling Protein 3 (UCP3)

Cat no: U1622-21

Uncoupling Protein 3 (UCP3)

Mitochondrial uncoupling proteins (UCP) are members of the larger family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. The different UCP's have tissue-specific expression; UCP3 is primarily expressed in skeletal muscle. It is postulated to protect mitochondria against lipid-induced oxidative stress. Expression levels of UCP3 increase when fatty acid supplies to mitochondria exceed their oxidation capacity, and the protein enables the export of fatty acids from mitochondria. UCP's contain the three solcar protein domains typically found in MACPs (1).\n\nApplications:\nSuitable for use in Western Blot, Flow Cytometry and Immunoprecipitation. Not recommended for use in Immunofluorescence or Immunohistochemistry. Other applications have not been tested. \n\nRecommended Dilutions: \nWestern Blot: 1:1000-1:10,000 detects a band at ~28kD\nImmunoprecipitation: 1:10-1:100\nFlow Cytometry: 1:10-1:100\nOptimal dilutions to be determined by the researcher. \n\nPositive Control: \nHuman Testis, A673 lysates\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.\n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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SPECIFICATIONS

Catalog Number

U1622-21

Size

100ul

Applications

FC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.2, 0.05% BSA, 0.01% sodium azide, 50% glycerol.

P Type

Mab

Purity

Supernatant

Additional Info

Recognizes human UCP3. Species crossreactivity: mouse, rat.

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