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Urokinase Plasminogen Activator Receptor (uPAR, CD87, uPAR-1, PLAUR (plasminogen activator receptor, urokinase type)

Cat no: U2610-03A

Urokinase Plasminogen Activator Receptor (uPAR, CD87, uPAR-1, PLAUR (plasminogen activator receptor, urokinase type)

CD87, is a glycosyl phosphatidylinositol-linked receptor which serves to localize and enhance the proteolytic activity of the urokinase-type plasminogen activator (uPA). The\nuPA/uPAR interaction also initiates signal transduction responses resulting in activation of protein tyrosine kinases, gene expression, cell adhesion and chemotaxis.\n\nApplications:\nSuitable for use in ELISA, Western Blot and Flow Cytometry.\nOther applications have not been tested. \n\nRecommended Dilutions: \nBlockade of Receptor-Ligand Interaction: ~0.5-2.5ug/ml. Blocks 50% of the binding of 50ng/ml of rhUrokinase to immobilized human rmuPAR (100ul of\na 0.5ug/ml solution was coated in each well) in a functional ELISA assay.\nELISA (direct): 0.5-1.0ug/ml. The detction limit is ~1ng/well.\nWestern Blot: 1-2ug/ml. The detection limit is 25ng/lane under non-reducing conditions. \nFlow Cytometry: 25ug/ml and add 10ul of the diluted solution to 1-2.5 x 10e5 cells in a total reaction volume not exceeding 200ul. The binding of unlabeled monoclonal antibodies may be visualized by adding 10ul of a 25ug/ml solution of a\nsecondary developing reagent such as goat anti-rat IgG conjugated to a fluorochrome.\nOptimal dilutions should be determined by each laboratory for each application.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Reconstitute to nominal volume by adding sterile buffer. Aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

U2610-03A

Size

500ug

Applications

ELISA, FC, WB

Hosts

Rat

Reactivities

Mouse

Form

Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 1ml sterile PBS.

P Type

Mab

Purity

Purified by Protein G affinity chromatography. Endotoxin: \n(same/less than) 0.1EU/ug of the antibody as determined by the LAL method.

Isotype

IgG2A

Additional Info

Recognizes mouse uPAR. Does not cross-react with rhuPAR in direct ELISAs or Western Blot.

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