Zic1 (Zinc Finger Protein of the Cerebellum 1, Zic Family Member 1 (Odd-paired Drosophila Homolog), Zic Family Member 1, Zic 1, Zic-1, ZIC, Odd Paired Homolog Drosophila, Zinc Finger Protein ZIC 1, Zinc Finger Protein ZIC1, ZNF201)

The Zic-1 gene, which encodes a zinc finger protein, is expressed in the developing or matured central nervous system in a highly restricted manner. The Zic gene is expressed in granule cells that make synaptic contact with Purkinje cells. Clearly Zic-1 is a gene critical to cerebellar pattern formation. The expression of Zic genes is first detected at gastrulation and at neurulation, becomes restricted to the dorsal neural ectoderm and the dorsal paraxial mesoderm. Zic-2 and Zic-3 are highly similar genes, especially in their product's zinc finger motif and by comparison of their genomic organization in that they share common exon-intron boundaries and belong to the same gene family. By comparison in function, Zic-2 is essential for the formation of the brain and Zic-3 is important for right and left axis formation. The Zic-1 gene has been mapped to chromosome 9 in mouse. The 5' flanking region of the Zic-1 gene contains a region-specific enhancer determined to be essential in in vivo and in vitro deletion analysis. The temporal profile of mRNA expression differs for each of the Zic gene products. The Drosophila odd-paired gene is highly homologous to the Zic gene family.\n\nApplications: \nSuitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: Mouse cerebellum\n\nCellular Localization: Nuclear\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 3 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

Z0421-81

Size

100ul

Applications

IF, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7

P Type

Pab

Purity

Purified

Isotype

IgG

References

Aruga, J., et al. (1996). The mouse zic gene family. Homologues of the Drosophila pair-rule gene odd-paired. J. Biol. Chem. 12;271(2): 1043-7.\nMikoshiba, K., (1999). Mamalian neural induction and brain pattern formation. Keio J. Med. Sep48(3): 111-23.\nNagai, T. et al., (1997). The expression of the mouse Zic1, Zic2, and Zic3 gene suggests an essential role for Zic genes in body pattern formation. Dev. Biol. Feb15;182(2): 299-313.\nAruga, J. et al., (2000). A 5' segment of the mouse zic1 gene contains a region specific enhancer for dorsal hindbrain and spinal cord. Brain Res. Mol. Brain Res.31;78(1-2): 15-25.

Additional Info

Detects a band of approximately 60kD. Species Crossreactivity: Crossreacts with Human and Mouse.