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ABO, Recombinant, Human, aa54-354, His-tag (Histo-blood Group ABO System Transferase, Fucosylglycoprotein 3-alpha-galactosyltransferase, Histo-blood Group A Transferase, Histo-blood Group B Transferase, NAGAT)

Cat no: 029646

ABO, Recombinant, Human, aa54-354, His-tag (Histo-blood Group ABO System Transferase, Fucosylglycoprotein 3-alpha-galactosyltransferase, Histo-blood Group A Transferase, Histo-blood Group B Transferase, NAGAT)

ABO, belongs to the glycosyltransferase 6 family. This protein is the basis of the ABO blood group system and related to the first discovered blood group system, ABO. Which allele is present in an individual determines the blood group. The histo-blood group ABO involves three carbohydrate antigens: A, B, and H. A, B, and AB individuals express a glycosyltransferase activity that converts the H antigen to the A antigen (by addition of UDP-GalNAc) or to the B antigen (by addition of UDP-Gal), whereas O individuals lack such activity.\n\nSource:\nRecombinant corresponding to aa54-354 from human ABO, fused to His-tag at N-terminal expressed in E.coli.\n\nAA Sequence:\nMGSSHHHHHH SSGLVPRGSH MAVREPDHLQ RVSLPRMVYP QPKVLTPCRK DVLVVTPWLA PIVWEGTFNI DILNEQFRLQ NTTIGLTVFA IKKYVAFLKL FLETAEKHFM VGHRVHYYVF TDQPAAVPRV TLGTGRQLSV LEVRAYKRWQ DVSMRRMEMI SDFCERRFLS EVDYLVCVDV DMEFRDHVGV EILTPLFGTL HPGFYGSSRE AFTYERRPQS QAYIPKDEGD FYYLGGFFGG SVQEVQRLTR ACHQAMMVDQ ANGIEAVWHD ESHLNKYLLR HKPTKVLSPE YLWDQQLLGW PAVLRKLRFT AVPKNHQAVR NP\n\nMolecular Weight:\n~37.4kD (MALDI-TOF)\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

029646

Size

50ug

Form

Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 2mM DTT, 20% glycerol, 200mM sodium chloride.

Purity

~85% (SDS-PAGE)

References

1. Persson M., et al. (2007) J. Biol. Chem. 282:9564-9570. 2. Nydegger, U.E., et al. (2005) Ann. N.Y. Acad. Sci. 1050: 40-51.

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