Activins belong to the TGF-beta superfamily. They are homo- or heterodimers of activin beta subunits. At least four mammalian beta subunits, A, B, C, and E, have been described. The beta c subunit homodimerizes to form Activin CC. A naturally occuring Activin AC heterodimer has also been reported.
Applications:
Suitable for use in Direct ELISA and Western Blot. Other applications not tested.
Recommended Dilutions:
Direct ELISA: 0.5-1.0ug/ml. The detection limit is ~10ng/well.
Western Blot: 1-2ug/ml. The detection limit is ~25ng/lane under non-reducing and reducing conditions.
Optimal dilutions to be determined by the researcher.
Hybridoma:
Mouse myeloma with B cells obtained from a mouse
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.