Adiponectin/CTRP1-7, Antibody Sampler Set, BioAssay(TM)

Adipose tissue of an organism plays a major role in regulating physiologic and pathologic processes such as metabolism and immunity by producing and secreting a variety of bioactive molecules termed adipokines. One highly conserved family of adipokines is adiponectin/ACRP30 and its structural and functional paralogs, the C1q/tumor necrosis factor- a-related proteins (CTRPs) 1-7. Unlike the CTRPs, which are expressed in a wide variety of tissues, adiponectin is reported to be expressed exclusively by differentiated adipocytes. These proteins are thought to act mainly on liver and muscle tissue to control glucose and lipid metabolism. Adiponectin is present in high levels in normal human plasma, but is reduced in obese subjects and often in those with increased insulin resistance and type 2 diabetes, suggesting that adiponectin may be a useful pharmacological target in various metabolic diseases. An analysis of the crystal structure of adiponectin revealed a structural and evolutionary link between TNF and C1q-containing proteins, suggesting that these proteins arose from a common ancestral innate immunity gene.\n\nRabbit polyclonal antibodies were raised against peptide sequences corresponding to each of the target proteins.\n\nKit Components:\nAdiponectin, CT (Rb x Hu): 1x25ug\nCTRP1, NT (Rb x Hu): 1x25ug\nCTRP2, NT (Rb x Hu): 1x25ug\nCTRP3, ID (Rb x Hu): 1x25ug\nCTRP4, CT (Rb x Hu): 1x25ug\nCTRP5, NT (Rb x Hu): 1x25ug\nCTRP6, CT (Rb x Hu): 1x25ug\nCTRP7, CT (Rb x Hu): 1x25ug\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-2ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nMouse Heart Tissue Lysate\nHeLa Whole Lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A0884-80M

Size

1 Set

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide.

References

1. Fantuzzi, G. et al. J. Allergy Clin. Immunol. 115, 911 (2005). 2. Tsao, T.S. et al. Euro. J. Pharmacol. 440, 213 (2002). 3. Wong, GW. et al. Proc. Natl. Acad. Sci. USA 101, 10302 (2004). 4. Lihn, AS. et al. Obes. Rev. 6, 13 (2005). 5. Shapiro, L. et al. Curr. Biol. 8, 335 (1998).