Adrenergic Receptor Kinase, beta 1, phosphorylated (Ser670) (bARK1, B1 AR, ARK1 beta, G Protein Coupled Receptor Kinase 2, GPCR K2, GRK2)

GRK2, one of six members of this family that have been identified, is ubiquitously expressed in mammals. After binding to their ligand and interacting with heterotrimeric G proteins, GPCRs (e.g., b2-adrenergic receptor) are phosphorylated by GRKs. Internalization of the GPCRs, regulated by b-arrestin-1, leads to activation of the Ras ? Raf ? ERK1&2 signaling pathway. GRK2 activity is tightly controlled by different mechanisms including phosphorylation by kinases such as PKC, Src and ERK1&2, as well as interaction with various proteins. ERK phosphorylates and thus inactivates GRK2 on serine 670 in a negative feedback mechanism.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.1-1 ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nPC12 and CHO-K cells.G protein-coupled receptor kinases (GRKs) are important regulators of G protein-coupled receptors (GPCRs). \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A0906-96

Size

10 Blots

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

1. Dalle, S., et al. (2002) Insulin induces heterologous desensitization of G protein-coupled receptor\nand insulin-like growth factor I signaling by downregulating beta-arrestin-1. Mol. Cell. Biol. 22(17):6272-6285. 2. Penela, P., et al. (2001) Beta-arrestin- and c-Src-dependent degradation of G-protein-coupled receptor kinase 2. EMBO J. 20(18):5129-5138. 3. Pitcher, J.A., et al. (1999) Feedback inhibition of G protein-coupled receptor kinase 2 (GRK2) activity by extracellular signal-regulated kinases. J. Biol. Chem. 274(49):34531-34534. 4. Aragay, A.M., et al. (1998) G protein-coupled receptor kinase 2 (GRK2): mechanisms of regulation and physiological functions. FEBS Lett. 430(1-2):37-40. 5. Lefkowitz, R.J. (1998) G protein-coupled receptors. III. New roles for receptor kinases and betaarrestins in receptor signaling and desensitization. J. Biol. Chem. 273(30):18677-18680.

Additional Info

Recognizes human GRK2 when phosphorylated at Ser670. Species Crossreactivity: Rat GRK2. Species sequence homology: mouse, hamster and bovine (100%), GRK2, GRK3 (64%).