AF4, NT (MLLT2, Mixed Lineage Leukemia, Translocated to, 2, ALL1 fused gene from chromosome 4, AF4, FEL)

MLLT2 is involved in acute leukemias through a chromosomal translocation t(4;11)(q21;q23) that involves mllt2 and mll/hrx. AF-4 (MLLT2), AF-9, and ENL proteins contain nuclear targeting sequences as well as serine-rich and proline-rich regions. Stretches abundant in basic amino acids are also present in the three proteins. These results suggest that the different proteins fused to ALL-1 polypeptide(s) provide similar functional domains. AF4 is a serine- and proline-rich putative transcription factor with a glutamine-rich carboxyl terminus. The composition of the complete MLL-AF4 fusion product argues that it may act through either a gain-of-function or a dominant negative mechanism in leukemogenesis.\n\nApplications:\nSuitable for use in ELISA\n\nRecommended Dilution:\nELISA: 1:1,000\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

031672

Size

200ul

Applications

ELISA

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

P Type

Pab

Purity

Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.

Isotype

IgG

References

Bursen, A., et al., Oncogene 23(37):6237-6249 (2004).\nBeausoleil, S.A., et al., Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135 (2004).\nCaslini, C., et al., Leukemia 18(6):1064-1071 (2004).\nBertrand, F.E., et al., Leukemia 17(12):2454-2459 (2003).\nReichel, M., et al., Leukemia 15(2):286-288 (2001).

Additional Info

Human

Alternative Names

MLLT2, Mixed Lineage Leukemia, Translocated to, 2, ALL1 fused gene from chromosome 4, AF4, FEL