AHR (Aryl-hydrocarbon receptor; also bHLHE76) is a 110kD member of the bHLH/PAS transcription factor family. It is widely expressed (breast, lung, liver), and serves many functions. First, it binds multiple xenobiotic chemicals in the cytoplasm. This induces dimerization with ARNT, translocation to the nucleus, and activation of P450 genes such as CYP1A1 and UGT1A6. Second, it appears to block cell cycle progression, possibly via a downregulation of CDK proteins. And third, it blocks apoptosis by interacting with E2F1, thus silencing TP73 and Apaf1 genes. Human AHR is 848aa in length. It contains a 10aa prosegment, plus a 838aa mature molecule that contains a DNA binding motif (aa13-40), a bHLH region (aa41-81), and two PAS domains (aa111-342). Over aa704-848, human AHR shares 70% aa identity with mouse AHR.
Applications:
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution:
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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