Annexin A11 (ANX11, ANXA11, Calcyclin-associated Annexin 50, CAP50)

Annexin A11 (ANXA11), also known as Annexin XI, 56K Autoantigen and Calcyclin-associated annexin 50 (CAP50), is a 54.4kD (predicted) member of the Annexin protein family. The Annexins are a family of Calcium-dependent phospholipid-binding proteins that are preferentially located on the cytosolic face of the plasma membrane. The Annexin's have a molecular weight of approximately 35-40kD and consist of a unique amino terminal domain followed by a homologous C-terminal core domain containing the calcium-dependent phospholipid-binding sites. The C terminal domain is comprised of four 60-70 amino acid (aa) repeats, known as annexin repeats or an endonexin fold (Annexin A6 contains 8 annexin repeats). The four annexin repeats form a highly a-helical, tightly packed disc known as the annexin domain, which binds to phospholipids in the membrane in a calcium-dependent manner. Members of the annexin family play a role in cytoskeletal interactions, phospholipase inhibition, regulation of cellular growth, and intracellular signal transduction pathways. Annexin A11 was identified as a 56 kD antigen recognized by sera from patients with autoimmune diseases such as rheumatoid arthritis, systemic lupus erythematosus, or Sjogren's syndrome. Human Annexin A11 shares 93% identity with mouse and 49% identity with rat Annexin A11. \n \nApplications:\nWestern Blot: An antibody concentration of 1.0ug/ml is recommended. \nOptimal dilutions should be determined by each laboratory for each application. \n \nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A2295-15K

Size

100ug

Applications

WB

Hosts

Goat

Reactivities

Hum, Mouse, Rat

Form

Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.1ml is used, the concentration will be 1mg/ml.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

Additional Info

Detects endogenous human, mouse, and rat Annexin A11 on Western Blots. Does not crossreact with recombinant human Annexin A1, A2, A3, A4, A5, A6, A7, A8, A9, A10, or A13 in Western Blots.