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ATP-Citrate Lyase (ACL, ATP Citrate (pro-S) Lyase, ATP Citrate Synthase, Citrate Cleavage Enzyme, EC 2.3.3.8), (CT)

Cat no: A4001-35B

ATP-Citrate Lyase (ACL, ATP Citrate (pro-S) Lyase, ATP Citrate Synthase, Citrate Cleavage Enzyme, EC 2.3.3.8), (CT)

ATP citrate lyase (ACL) is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer of four identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. One of these products, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. NDPK has been found to phosphorylate ACL and insulin to increase phosphorylation of ACL (2).\n\nApplications:\nSuitable for use inFlow Cytometry, Western Blot, Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1,000-1:5,000\nFlow Cytometry: 1: 30\nImmunoprecipitation: 1:50\n\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. \n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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SPECIFICATIONS

Catalog Number

A4001-35B

Size

100ul

Applications

FC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. Elshourbagy et al. Europ. J. Biochem. 204: 491-499, 1992. 2. Benjamin, W. B., and Singer, I. (1974) Biochim. Biophys.Acta 251, 28-42.

Additional Info

Recognizes human ATP-Citrate Lyase.

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