b-Amyloid 1-40, 42

Alzheimer's Disease (AD) is a neurodegenerative disorder characterized by progressive loss of memory and cognition in the elderly. One of the most important and initial step involves proteolytic cleavage of amyloid precursor protein (APP, chromosome 21) releasing short 40, 42 & 43aa peptides (beta amyloid1-40, 1-42 and 1-43). Polymerization of b-amyloid (Ab) and subsequent neuronal deposit (amyloid) leads to the degeneration of neurons involved in memory and cognition. Ab deposits have also been found to contain 2 additional proteins termed a-synuclein and b-synuclein. The 140aa a-synucleins is identical with non- Ab component (NACP) of AD. The 134aa human b-synuclein is homologous to 14kD bovine phosphoneuroprotein 14. Mutations in a-synuclein gene causing a replacement of alanine with a threonine may cause the protein to misfold. Synucleins are primarily expressed in the brain. At least 3 forms: two large (140 aa SYN-1 & 149 aa SYN-2) and a small form (SYN-3, 42 aa) are produced by alternative splicing.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well. \nWestern Blot: 1:1000-1:5000 (ECL).\nOptimal dilutions to be determined by the researcher.\n\nRecommended Control Peptide:\nFull length b-amyloid (1-40aa and 1-42aa).\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A2275-75K

Size

100ul

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a lyophilized powder, 0.05% sodium azide. Reconstitute with 100ul sterile PBS.

P Type

Mab

Purity

Ascites

Isotype

IgG1

References

1. Neve R et al (1992) PNAS 89, 3448. 2. Goldgaber D et al (1987) Science 235, 877. 3. Yankner B et al (1989) Science 245, 417. 4. Golde T et al (1992) Science 255, 728. 5. Wang R et al (1991) J. Biol. Chem. 266, 16960. 6. Shigematuse K et al (1992) J Neurosci. 31, 443. 7. Kang J et al (1987) Nature 325, 733. 8. Tanzi r et al (1987) Science 235, 880. 9. Weidmann A et al (1989) Cell 57, 115.

Additional Info

Recognizes human b-amyloid1-40/42. Species Sequence Homology: mouse, rat and many other species.