BNC1 (Zinc Finger Protein Basonuclin-1, BNC)

Bnc 1 is a 994aa transcription factor specific for squamous epithelium and for the constituent keratinocytes at a stage either prior to or at the very beginning of terminal differentiation. It is a zinc finger protein with three separated pairs of zinc fingers and a nuclear localization signal. Bnc 1 is a soluble protein that can shuttle between the nucleus and the cytoplasm, and its location depends on the proliferative potential of the cell. It is expressed relatively uniformly in the nucleoplasm, and phosphorylation on Ser-537 and Ser-541 leads to its cytoplasmic localization. It is present in the basal cell layer of the epidermis, in hair follicles and also in abundance in the germ cells of testis and ovary, and to a lower extent in thymus, spleen, mammary glands, placenta, brain and heart. Reports suggest that it plays a regulatory role in keratinocyte proliferation and also in rRNA transcription. It is also known to play a role in the differentiation of spermatozoa and oocytes.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 4-7ug/ml\nOptimal dilutions to be determined by the researcher. \n\nPositive Control:\nHuman and rat spleen lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

B0132-05

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, NHP

Form

Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.

P Type

Pab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG

References

1. Vanhoutteghem, A. et al. Proc. Natl. Acad. Sci. USA. 103:12423-12428 (2006). 2. Tseng, H. et al. J. Cell. Sci. 18:3039-3047 (1999). 3. Tseng, H. et al. Proc. Nat. Acad. Sci. 89:10311-10315 (1992).

Additional Info

Recognizes human BNC1. Species Crossreactivity: chimpanzee, monkey. Species sequence homology: bovine, equine, mouse, rat, canine.