BNIP3 (Bcl2/Adenovirus E1B 19kD Protein-Interacting Protein 3, 19kD Interacting Protein 3, NIP3)

BNIP3 is a pro-apoptotic, mitochondrial protein classified in the Bcl 2 family. BNIP3 expressed in yeast and mammalian cells interacts with survival promoting proteins Bcl 2, Bcl XL, CED9 and the adenovirus E1B 19K protein. BNIP3 represents a subfamily of Bcl 2 related proteins, which function without a typical BH3 domain to regulate apoptosis from both mitochondrial and nonmitochondrial sites by selective Bcl 2/Bcl XL interactions. The N-terminus (aa1-49) and the C-terminus TM domain of BNIP3 are critical for Bcl 2 hetero- dimerization, and either region is sufficient for Bcl XL interaction. The TM domain of BNIP3 is critical for homodimerization, pro-apoptotic function and mitochondrial targeting. BNIP3 contains PEST sequences suggesting that the protein may be susceptible to rapid degradation by proteases. The post- translational control of BNIP3 expression through rapid protein degradation may constitute a mechanism for regulating the intracellular levels of a potentially lethal protein. Homologues of BNIP3 sharing both structural and functional similarity have been identified in mammals (Nix) and, in C. elegans (ceBNIP3). Endogenous BNIP3 is loosely associated with mitochondrial membrane in normal tissue but fully integrates into the mitochondrial outer membrane with the N-terminus in the cytoplasm and the C-terminus in the membrane during induction of cell death. This is accompanied by rapid and profound mitochondrial dysfunction. Endogenous BNIP3 protein is abundant in murine andhuman skeletal muscle and is not detectable in lysates of all other nonskeletal muscle-bearing tissues and many cell lines, including myoblasts and differentiated myocytes.\n\nApplications: \nSuitable for use in ELISA, Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.\nRecommended Dilution:\nWestern Blot: 2-4ug/ml. Detects a band at ~30kD and ~60kD\nOptimal dilutions to be determined by the researcher.\nPositive Control: Whole cell lysate from 293T cells transfected with BNIP3\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

B2199-97B

Size

200ul

Applications

ELISA, ICC, IP, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS. pH 7.4, 15mM sodium azide.

P Type

Mab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG2b

References

1. Ray, R., et al., J. Biol. Chem., 275, 1439-1448 (2000).2. Vande Velde, C., et al., Mol. Cell. Biol., 20, 5454-5468 (2000).3. Chen, G., et al., J. Exp. Med., 186, 1975-1983 (1997).4. Reed, J.C., Am. J. Pathol., 157, 1415-1430 (2000).5. Yasuda, M., et al., J. Biol. Chem., 273, 12415-12421 (1998).6. Cizeau, J., et al., Oncogene, 19, 5453-5463 (2000).7. Yasuda, M., et al., Cancer Res., 59, 533-537 (1999).8. Chen, G., et al., J. Biol. Chem., 274, 7-10 (1999).

Additional Info

Recognizes human BNIP3.